Identification of disease-causing mutations in DMD gene of Duchenne muscular dystrophy

VernacularTitle:假肥大型肌营养不良症患者及携带者的致病基因突变检测
Author: Ben-Chang SHEN ¹ ; Quan-Xi SU ; Shan-Wei FENG ; Ying-Yin LIANG ; Cheng ZHANG
Author Information

1. 广州医学院
Keywords: Duchenne muscular dystrophy; Deletion; Duplication; Point mutation; Carrier
From: Chinese Journal of Neuromedicine 2008;7(6):581-584
CountryChina
Language:Chinese
Abstract: Objective To detect the disease-causing mutations in Duchenne muscular dystrophy (DMD) gene of DMD or Becher's muscular dystrophy (BMD) patients or carriers. Methods Multiplex ligation-dependent probe amplification (MLPA) and denaturing high performance liquid chromatography (DHPLC) were coupled to analyze the disease-causing mutations in DMD gene. Results Ten patients were detected to have deletions in different exons; 1 patient was caused by duplication of exon 50 using DHPLC analysis, and 4 patients were found to be caused by non-sense point mutations. However, the disease-causing mutations of other 5 patients remained to be determined. Conclusion MLPA coupled with DHPLC analysis can be used to detect the disease-causing mutations of DMD or BMD systematically, and provide valuable information for the affected families in preventing from recurrence of DMD or BMD.