Modulation of proton-gated current by substance P in trigeminal ganglion neurons of rat
10.3760/cma.j.issn.1671-8925.2008.06.006
- VernacularTitle:P物质对大鼠三叉神经节神经元H+-门控电流的调制
- Author:
Jian ZHANG
1
;
Mei LI
;
Chun-Yuan LI
;
Zhi-Wang LI
;
Bin ZHAO
;
Hong-Ling WU
;
Zhuo-Wa SU
;
Hong PANG
Author Information
1. 广东省深圳市南山医院
- Keywords:
Acid-sensing ion channels;
Proton-gated current;
Substance P;
Trigeminal ganglion;
Modulation
- From:
Chinese Journal of Neuromedicine
2008;7(6):562-566
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the modulatory effect of substance P (SP) on proton-gated current in the membrane of rat trgeminal ganglion (TG) neurons and its underlying mechanism. Methods Neurons were isolated mechanically and enzymatically from TG of rat. Whole-cell patch clamp technique was used for recording the proton-gated current in freshly isolated neurons. Results Proton-gated currents recorded from rat TG neurons could be classified into 4 distinct types: T-type, S-type, B-type and O-type in the present study. Co-application of SP and proton potentiated S-type proton-gated currents in a concentration-dependent manner, and the potentiation was not blocked by SP receptor antagonist, GR82334; co-application of SP and proton potentiated B-type proton-gated currents, and GR82334 and intracellular dialysis of GDP-β-S blocked the potentiation of SP. Pre-application of SP inhibited B-type proton-gated current, especially the transient component. The inhibition could not be reversed by pretreatment wit-h GR82334. Conclusions The mechanisms of modulation of proton-gated current by SP is associated with the difference of their makeup of subunits of acid-sensing ion channels (ASICs), and there may be an allosteric position of SP in the outside framework of ASICs in neuronal membrane.
