Effects of coriaria lactone-activated astrocyte conditioned medium on the expressions of glutamate and GluR2 in the rat brain
10.3760/cma.j.issn.1671-8925.2008.06.003
- VernacularTitle:马桑内酯激活的星形胶质细胞条件培养液对大鼠脑内谷氨酸及其受体GluR2表达的影响
- Author:
Shu-Hua ZHANG
1
;
Chang-Geng ZHU
;
Qing-Ying LIU
;
Ying WEI
;
Wei WANG
Author Information
1. 滨州医学院
- Keywords:
Astrocyte;
Conditionedmedium;
Coriarialactone;
Glutamate;
Receptor
- From:
Chinese Journal of Neuromedicine
2008;7(6):549-553
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of coriafia lactone (CL)-activated astrocytes (Ast) conditioned medium (ACM) on the expressions of glutamate (Glu) and GluR2 in the brain of rat. Methods Asts of hippocampus were cultured according to the McCarthy and DeVellis's method, and then the ACM was collected. Forty-eight male adult Sprague-Dawley (SD) rats were randomly divided into the control group (n=16) and the CL group (n=32). Rats in the control group were administered 10 μL ACM I. C. V., which was not added any stimulating substance. Rats of the CL group were injected I. C. V. 10 μL CL-activated ACM. The rats in both groups were subdivided into post-injection 2,4,8,12h subgroups, 4 in each subgroup in the control group and 8 in each subgroup in the CL group. The behaviors of the rats were observed and the expressions of Glu and GluR2 in the cerebral cortex and hippocampus were detected with immunohistochemistry and immunofluorescence. The content of GluR2 was tested with Western blot. Results The rats injected with CL-activated ACM showed seizure activities, whereas the rats of the control group showed no seizure activities. The expression of Glu in cerebral cortex and hippocampus in the brains injected with CL-activated ACM was increased compared with the control group at 4h (P<0.05), but the expression of GluR2 was attenuated compared with the control group at 4h(P<0.05). The results of GluR2 in the cerebral cortex and hippocampus detected with Western blot were different significantly with control group (P<0.05). Conclusion CL-activated ACM can enhance the expression of Glu and reduce the expression of GluR2 in the brain of rat, resulting in the activation of AMPA pathway and the Ca2+ influx, and then induce seizure activities.
