Diagnosis of Duchenne muscular dystrophy through dystrophin expression detection by immunohistochemistry
10.3760/cma.j.issn.1671-8925.2008.05.021
- VernacularTitle:免疫组织化学dystrophin染色诊断Duchenne型肌营养不良症的研究
- Author:
Peng LIU
1
;
Ding-Guo SHEN
Author Information
1. 解放军总医院
- Keywords:
Duchenne;
Dystrophin;
Immunohistochemistry
- From:
Chinese Journal of Neuromedicine
2008;7(5):508-510
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study dystrophin expression in Duchenne muscular dystrophy (DMD) and non-DMD patients. Methods With immunohistochemistry method, using monoclonal antibody of dystrophin, expression of dystrophin was analyzed in 7 DMD patients (experimental group)and 7 non-DMD patients (control group). Results In 7 non-DMD patients, uniform and continuous dystrophin expression was found along the sarolemma, while not in 7 DMD patients. Conclusions Dystrophin expression in myocyte membrane is negative in DMD patients, which indicates that dystrophin loss may be involved in the pathogenesis of DMD. It can be used as a "gold standard" in diagnosing DMD.
