Comparison of epilepsy models reproduced by penicillin and kainic acid
10.3760/cma.j.issn.1671-8925.2008.05.008
- VernacularTitle:青霉素与海人酸癫痫模型的比较
- Author:
Xiao-Gang YANG
1
;
Wei-Ping LIU
;
Xiang ZHANG
;
Jian-Bo WANG
;
Xi-Tuan JI
Author Information
1. 第四军医大学西京医院
- Keywords:
Penicillin;
Kainic acid;
Epilepsy;
Mice;
Models
- From:
Chinese Journal of Neuromedicine
2008;7(5):461-463
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the mouse models of acute epilepsy induced by kainic acid or penicillin, and explore the characteristics and condition of application of the 2 agents. Methods Ninety healthy male Kunming mice were selected and divided randomly into the normal saline group (n=10) and penicillin-induced group (n=40) and kainic acid-induced group (n=40). The kainic acid-induced group were treated with 10 mg/kg kainic acid by intraperitoneal injection, the penicillin-induced group were treated with 7× 106 U/kg penicillin by intraperitoneal injection, and the ones in the normal saline group were treated with 35 μL/g normal saline by intraperitoneal injection. After injection, seizure was observed continuously and graded in the following 5 h and monitored by electroencephalogram (EEG). Results Totally 80 mice of the penicillin- and kainic acid-induced groups entered the final analysis. It was noted that the latency of the appearance of the status epilepticus by kainic acid was shorter than that by penicillin (P＜0.05), and the mortality rate was lower as well (P＜0.05). Conclusions Acute epilepsy model reproduced by kainic acid might be superior to the one by penicillin because of the similar behavior and EEG features with human temporal epilepsy.
