Effects of human urinary kallikrein on cell apoptosis of rats with acute focal cerebral ischemia-reperfusion injury
10.3760/cma.j.issn.1671-8925.2008.03.016
- VernacularTitle:人尿激肽原酶对急性局灶性脑缺血再灌注损伤大鼠细胞凋亡的影响
- Author:
Ling-Li LU
1
;
Zhen-Hua LIU
;
Hui-Fang XIE
;
Duo-Bin WU
;
Xiao-Ya GAO
Author Information
1. 南方医科大学珠江医院
- Keywords:
Focal cerebral ischemia-reperfusion injury;
Human urinary kallikrein;
Apoptosis
- From:
Chinese Journal of Neuromedicine
2008;7(3):273-277
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of human uriilary kallikrein(HUK)on the number of apoptotic cells and the expressions of Bcl-2 and Bax proteins in rats after focal cerebral ischemia and reperfusion(FCIR) injury. Methods Eighty-four Spmque-Dawley(SD)male rats were randomly divided into sham-operated group(n=12),ischemia-reperfusion group(n=36),and HUK-treated group (n=36). Transient focal cerebml ischemia models were established by middle cerebml artery occlusion.Six rats were chosen from sham-operated group,ischemia-reperfusion group,and HUK-treated group for measuring infarct sizes.The rest were used to evaluate neurologic fhnction impaiment and measure the nunlber of apoptotic cells and Bcl-2 or BaX protein positive cells in cerebral cortex with TUNEL and immunohistochemistry.The latter 2 groups were subdivided into 6,12,24,72,168 h reperfusio groups (each n=6). Results The neurologic function impairmlent score,the infarct sizes,the apoptotic cells and the expression of Bax protein of HUK-treated group at different time points (except 168 h group)significantly decreased compared wilh those of ischemia-reperfsion group (p＜0.05).The expression of Bcl-2 protein of HUK-treated group at different time points(except 168 h group) remarkably increased compared with that of ischemia-reperfusion group(P＜0.05). Conclusions HUK can excrt a protection against FCIR injury, maybe through up-regulating Bcl-2 and down-regulating Bax protein in the initial 3 d of FCIR injury to decrease the number of apoptotic cells
