Increased expression of ERp29 in retinoic acid-induced PC12 cells by mutant presenilin-1 and its significance
10.3760/cma.j.issn.1671-8925.2008.03.011
- VernacularTitle:突变型早老素-1致维甲酸诱导PC12细胞ERp29表达增强及其意义
- Author:
Rong-Hua YANG
1
;
Qi-Lin HUANG
Author Information
1. 第三军医大学附属新桥医院
- Keywords:
Alzheimer's disease;
Presenilin-1;
Endoplasmic reticulum protein 29
- From:
Chinese Journal of Neuromedicine
2008;7(3):250-253,257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of mutant presenilin-1(PS-1)on retinoic acid (RA)-induced PC12 cell gene expression,and find out the pathogenesis-related genes of Alzheimer's disease. Methods Gene-engineered PC12 cells stably expressing mutant PS-1 or wild-type PS-1 gene were induced by RA and then investigated by silver staining difrerential display (DD) technique for detecting gene differential expression. Results The gene-engineered PC12 cell models expressing mutant or wide-type PS-1 gene were successfully established by Lipofectin transferring technique. The endoplasmic reticulum protein 29 (ERp29) was higher expressed in gene-engineered PC12 cells with mutant PS-1,moreover,it had a close relationship with cell apoptosis. Conclusions The increase of ERp29 expression may be result from endoplasmic reticulum stress.The higher expression of ERp29 may play an important role in pathogenic mechanism of Alzheimer's disease.
