Time course changes of calpain activity following FPI to rats' neurons and the effect of mild hypothermia on calpain activity
10.3760/cma.j.issn.1671-8925.2007.06.011
- VernacularTitle:大鼠神经元液压伤后依钙蛋白酶的时程变化及亚低温的影响
- Author:
Li-Sen SUI
1
;
En-Zhong LIU
;
Xiao-Dan JIANG
;
Ru-Xiang XU
;
Fu HAN
;
Xiang-Tong ZHANG
;
Qin-Shun DAI
Author Information
1. 广州中医药大学附属第二医院(广东省中医院)
- Keywords:
Neuron;
Fluid percussion injury;
Calpain;
Mild hypothermia
- From:
Chinese Journal of Neuromedicine
2007;6(6):579-584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate time course changes of intraneuronal calpain activity following fluid percussion injury(FPI)under normothermia(37℃)and mild hypothermia(32+0.5)℃.Methods Rats' neurons produced by external culture and FPI models prepared, mild hypothermia was applied for intervention at different time points and the changes in intraneuronal calpain activity following FPI and the intervention effect of mild hypothermia on calpain activity during different time courses were detected by UV-spectrophotometry. Results Remarkable changes occurred to calpain activity following FPI at 37℃. While the intervention effect of mild hypothermia on calpain activity was different, closely related to the starting time point of intervention. Conclusion The changes of intraneuronal calpain activity following FPI join the pathological process of cellular trauma; mild hypothermia might protect patients with traumatic brain injury(TBI)to some extent through the regulation of calpain activity. And, the intervention effect of mild hypothermia has some correlation with the starting time point of intervention.
