Expression of water channel aquaporin-4 following status epilepticus in rats

VernacularTitle:大鼠癫痫持续状态后水通道蛋白-4的表达
Author: Lan-Qing MENG ¹ ; Shi-Ge WEI ; Rui-Ya HUANG ; Xue-Bin LI ; Ye-Sheng WEI
Author Information

1. 右江民族医学院附属医院
Keywords: Status epilepticus; AQP4
From: Chinese Journal of Neuromedicine 2007;6(2):144-147
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of water channel aquapoin-4 (AQP4) and its relationship to brain edema formation after status epilepticus (SE) in rats. Methods Fifty-four Sprague-Dawley rats (weighing 250～300 g) were randomly divided into 9 groups (n=6 in each group): the control group and the post-SE 6, 12, 24, 48, 72, 96, 120, 168 h groups. SE models were established with Lithium-pilocarpine. Immunohistochemistry and semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) were used to assess AQP4 protein and mRNA expression following SE. Results AQP4 expression significantly increased at 24 h, reached to a peak level at 48 h, lasted for 3 d and then began to decline, but still existed at day 7 (P＜0.05 vs control group). The time-dependent change displayed an obvious positive correlation with the process of brain edema after SE (r=0.73, P＜0.05).Conclusion The time-related change of AQP4 expression after SE has a positive correlation with brain edema, indicating that AQP4 may play an important role in the formation of brain edema following SE.