Study on hyperosmotic regulation-involved reciprocal pathways between medullary visceral zone and hypothalamic paraventricular nucleus or supraoptic nucleus
10.3760/cma.j.issn.1671-8925.2006.12.005
- VernacularTitle:大鼠延髓内脏带与下丘脑室旁核、视上核往返投射通路参与高渗调节反应的研究
- Author:
Rao WANG
1
;
Ping PENG
;
Zhi-Jun YANG
;
Ru-Xiang XU
;
Zhi-Ren RAO
;
Li DUAN
;
Xiao-Dan JIANG
Author Information
1. 南方大学珠江医院
- Keywords:
Neuron;
Astrocyte;
Osmotic pressure;
Glial fibrillary acidic protein;
Rat
- From:
Chinese Journal of Neuromedicine
2006;5(12):1199-1204
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the possibility of reciprocal pathways between medullary visceral zone (MVZ) and hypothalamic paraventricular nucleus (PVN) or supraoptic nucleus (SON) following hyperosmotic stimulation. Methods Hyperosmotic pressure animal model was established by administering3% sodium chloride as drinking water to rats. The distributions and expressions ofHRP retrogradely labeled neurons, Fos, tyrosine hydroxylase (TH) or vasopressin (VP) positive neuron and lial fibrillary acidic protein (GFAP) positive astrocytes (AST) in MVZ, SON and PVN were observed by quadruple labeling methods of WGA-HRP retrograde tracing combined with anti-Fos, TH (or VP) and GFAP immunohistochemical technique. Results Fos positive neurons within the MVZ, PVN and SON increased markedly. There were also a large number of GFAP positive structures in the brain and their distribution pattern was fundamentally similar or analogous to Fos positive neurons in the above-mentioned areas. The augmented GFAP reactivities took on hypertrophic cell bodies, thicker and longer processes.Quadruplicate immunohistochemical staining showed that a neuron could be closely surrounded by many AST and they formed neuron-astrocytic complex (N-ASC). Conclusion The neurons and AST might be very active following hyperosmotic pressure and N-ASC as a functional unit might serve to modulate the osmotic pressure. There was reciprocal osmoregulation pathways between the MVZ and SON or PVN in the brain.
