Photodynamic effect mediated with 5-aminolevulinic acid on U251 human glioma cells
10.3760/cma.j.issn.1671-8925.2006.01.011
- VernacularTitle:5-氨基乙酰丙酸介导的光动力对人脑胶质瘤细胞的治疗作用
- Author:
Lian-Shu DING
1
;
Ru-Xiang XU
;
Xiao-Dan JIANG
;
Yi-Gang YU
;
Tao HUANG
;
Jun YUAN
;
Zhong XU
;
Zhen-Zhou CHEN
;
Ying-Qian CAI
;
Yu-Xi ZOU
;
Mou-Xuan DU
Author Information
1. 南京医科大学附属淮安市第一人民医院
- Keywords:
Glioma;
5-aminolevulinic acid;
Photodynamic therapy;
U251
- From:
Chinese Journal of Neuromedicine
2006;5(1):38-41,48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the photodynamic effect mediated with 5-aminolevulinic acid (5-ALA) on U251 human glioma cells. Methods Fluorescence microscope and confocal laser scanning microscope were used to detect the localization of Pp Ⅸ in U251 human glioma cells. The cells with/without 5-ALA were irradiated at the wavelength of 635 nm. MTT assay was used to measure the cell survival after laser irradiation. Results 5-ALA cocultured with U251 cells successfully produced endogenous Pp Ⅸthat was observed distributively in the cytoplasm, but not in nuclear region. The overall survival rates of the U251 glioma cells photodamaged by ALA-PDT decreased as the incubation time went by or the 5-ALA concentration increased, while peaked at the incubation time of 6 h and the 5-ALA concentration of 2.0mmol/L. Without one of 5-ALA and light irradiation, the survival rate of the cells had no significant difference compared with that of cells of the control group. Conclusion The 5-ALA-induced PDT appears to be a promising therapy for human glioma. The optimal incubation time may be 6 h and the optimal 5-ALA concentration be 2.0 mmol/L.
