Effects of cannabidiol on the expression of Occludin and ZO 1 and the permeability of blood brain barrier in the cerebral cortex of rats with traumatic brain injury
10.19405/j.cnki.issn1000-1492.2024.03.017
- Author:
Jiali Li
1
;
Yan Cao
1
;
Tenghan Ling
1
;
Aiping Yin
1
;
Hengxi Li
1
;
Jinghui Li
2
;
Ruilin Zhang
3
;
Haiying Wu
4
;
Ping Li
1
Author Information
1. Dept of Human Anatomy and Histology and Embryology, Kunming Medical University , Kunming 650500
2. Dept of Neurosurgery, First Afiliated Hospital of Kunming Medical University , Kunming 650032
3. School of Forensic Medicine, Kunming Medical University , Kunming 650500
4. Dept of Emergency, First Afiliated Hospital of Kunming Medical University , Kunming 650032
- Publication Type:Journal Article
- Keywords:
cannabidiol;
traumatic brain inj ury;
blood brain barrier;
tight j unction protein;
rats;
Occludin;
zonula occluden-1
- From:
Acta Universitatis Medicinalis Anhui
2024;59(3):478-483
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To ob serve the expression and trends of tight j unction proteins Occludin and zonula occluden-1 (ZO-1) in blood brain barrier (BBB) of rats with traumatic brain injury (TBI) , and to explore the intervention effect of cannabidiol (CBD) on the BBB .
Methods :The TBI model of rat was prepared by modified “Feeney free fall method ”and randomly divided into three groups : the sham operated group ( Sham group) , the model group (TBI + vehicle group) and the CBD intervention group ( TBI + CBD group) , with 24 rats in each group . Each group was subdivided into six time points : 8 h , 1 , 2 , 3 , 5 and 7 d after injury. The expression of Occludin and ZO-1 , which are closely related to the permeability of BBB , was detected by immunohistochemistry , immuno fluorescence staining and Western blot at different points . The permeability of BBB was detected by sodium fluores cein assay.
Results :The results of immunohistochemistry showed that compared with Sham group , the positive ex pression of Occludin and ZO-1 decreased with time after brain trauma ( P < 0.05) , and both reached the lowest level at 2 d . The expression levels of Occludin and ZO-1 were up regulated after 1 d of CBD intervention ( P < 0.05) . Immunofluorescence staining showed a similar trend to Western blot results , with Occludin and ZO-1 fluo rescence expression intensity and protein expression reduced after TBI compared with Sham group ( P < 0.05 ) . And the expression levels of Occludin and ZO-1 were up regulated after 2 d of CBD intervention (P < 0.05) . The results of fluorescein sodium experiment showed that the BBB integrity of brain tissue was destroyed after TBI , and the permeability increased after TBI (P < 0.01) . The permeability of BBB decreased after CBD intervention (P < 0.05) .
Conclusion :The expression of tight junction proteins O ccludin and ZO-1 decreases after TBI , and the permeability of BBB is disrupted , and CBD intervention reverses the disruption of the BBB by TBI .
- Full text:202406270829154362大麻二酚对创伤性脑损伤大鼠...水平及血脑屏障通透性的影响_李佳丽.pdf
