Study on alleviating alcoholic fatty liver with Milk derived pentapeptide PGPIP and its mechanism
10.19405/j.cnki.issn1000-1492.2023.11.002
- Author:
Jiujiu Huang
1
;
Qilong Geng
1
;
Anqi Li
1
;
Yide Qin
1
;
Nan Qi
1
Author Information
1. Dept of Biochemistry and Molecular Biology , School of Basic Medical Sciences , Anhui Medical University , Hefei 230032
- Publication Type:Journal Article
- Keywords:
PGPIP;
alcoholic fatty liver;
endoplasmic reticulum stress;
ATF4;
ATF6
- From:
Acta Universitatis Medicinalis Anhui
2023;58(11):1813-1818
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the effects of milk derived pentapeptide prolin⁃glycine⁃proline⁃isoleucine⁃pro⁃
line ( PGPIP) on chronic alcoholic liver injury in mice and its related molecular mechanism .
Methods :Forty C57BL/6 mice were randomly divided into control group , model group , glutathione(GSH) group , PGPIPN group .The mice model of chronic alcoholic fatty liver was established by 10 d ad libitum oral feeding with the Lieber⁃DeCarli(LD) ethanol liquid diet plus one binge . Drug intervention was given at the same time . Based on the reported RNA sequencing data in gene expression omnibus (GEO) database , the differential expression of liver endoplasmic reticulum stress⁃related genes was analyzed by cluster heat map . Liver hematoxylin⁃eosin (HE) staining was used to analyze the pathological effects of each treatment group on alcoholic liver injury in mice . Oiled Red O staining was used to analyze the effects of each treatment group on the accumulation of liver lipid droplets in mice . Transtransduction protein expression was detected by Western blot .
Results :The pathological examination of PGPIP group was similar to that of Control group , and the liver injury of mice was significantly reduced . The accumulation of lipid droplets in the liver of mice in the model group was manifested as mixed lipid droplets of different sizes , and PGPIP treatment significantly reduced the accumulation of liver lipid droplets induced by alcohol . PGPIP had significant effects on the PERK⁃eIF2α⁃ATF4 pathway and the expression of transcriptional activator 6 ( ATF6 ), Cleaved Caspase 3 protein .
Conclusion :Pentapeptide PGPIP can alleviate chronic alcoholic fatty liver and liver injury in mice . The mechanism may be attributed to reducing the accumulation of lipid droplets in hepatocytes , en⁃ doplasmic reticulum stress , and hepatocyte apoptosis .
- Full text:202406261724111780乳源五肽PGPIP缓解酒精性脂肪肝及其机制研究_黄九九.pdf
