The effect of calcium phosphate nanoparticles⁃mediated interference of LMO4 on skin squamous cells
10.19405/j.cnki.issn1000-1492.2023.11.001
- Author:
Minghua Xiang
1
;
Liyu Guo
2
;
Zhenzhen Tu
3
;
Yue Wang
4
;
Haisheng Zhou
3
Author Information
1. Dept ofApplied Physics , Anhui Medical University, Hefei 230032
2. Shenzhen Bay Laboratory, Shenzhen 518107
3. Dept ofBiochemistry and Molecular Biology, Anhui Medical University, Hefei 230032
4. School of Clinical Medicine , Anhui Medical University, Hefei 230031
- Publication Type:Journal Article
- Keywords:
calcium phosphate nanoparticles;
LMO4;
skin squamous cells;
cell proliferation;
cell cycle
- From:
Acta Universitatis Medicinalis Anhui
2023;58(11):1807-1812
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate lim domain protein 4 (LMO4) functions and mechanisms in regulating proliferation of skin squamous cells (A431) , the shRNAs targeted to human LMO4 were coated by calcium phosphate nanoparticles (NP) and transfected into A431 cells to inhibit LMO4 expression.
Methods :Reverse transcription
and quantitative polymerase chain reaction ( RT⁃qPCR) , immunohistochemistry analysis and Western blot were used to detect expression of the interest genes. The expression vectors with shRNA targeted to human LMO4 (NP/sh⁃L) were coated by the calcium phosphate nanoparticles , and transfected into A431 . The MTT assay was conducted to determine cell proliferation after transfected for 24 , 36 and 48 h. Cells were stained with propidium iodide and examined cell cycles by using flow cytometry.
Results : LMO4 expressed at higher levels both in the skin squacalcium phosphate nanoparticles and DNA was 10 ∶ 1 . There was no significant difference of transfection efficiency between the NP/sh⁃L and lipofection approaches. The MTT assay showed that silencing LMO4 inhibited proliferadown did not alter expression of CDK4 and cyclin D1 .
Conclusion:The calcium phosphate nanoparticles could bind and transfer the foreign DNA into the targeted cells with high efficiency. Silencing LMO4 decreased expression of cyclin E and CDK2 resulted in inhibition of cell proliferation.
- Full text:2024062617170891053磷酸钙纳米颗粒介导干扰LM...4对皮肤鳞癌细胞增殖的影响_项明华 (1).pdf
