LncRNA MALAT1 promoted myocardial cell damage in oxidative stress models by regulating miR-181a
10.19405/j.cnki.issn1000-1492.2024.03.014
- Author:
Lihua Zheng
1
;
Siyao Wang
2
;
Peng Li
1
Author Information
1. Dept of Cardiology , The Fifth Afiliated Hospital of Xinjiang Medical University , Urumqi 830011
2. Dept of Endocrinology , The Fifth Afiliated Hospital of Xinjiang Medical University , Urumqi 830011
- Publication Type:Journal Article
- Keywords:
MALAT1;
microRNA-181 a;
acute myocardial infarction;
cell apoptosis;
cell proliferation;
cardio myocyte injury
- From:
Acta Universitatis Medicinalis Anhui
2024;59(3):455-463
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the expression and mechanism of long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and microRNA 181 a ( miR 181 a ) in a myocardial cell oxidative stress model.
Methods :The expression of MALAT1 and miR 181 a in peripheral blood of 30 patients with acute myocardial infarction ( AMI group) and 30 healthy controls ( Normal group) was detected by qRT PCR. Pearson correlation analysis was performed to determine the correlation between MALAT1 and miR 181 a in AMI . The binding sites between MALAT1 and miR-181a were predicted using the lncBase online prediction database and validated by dual luciferase reporter assay. An oxidative stress model of myocardial cells was established by hydro gen peroxide (H2O2 ) treatment in AC16 human myocardial cell line . siRNA targeting MALAT1 ( si-MALAT) and negative control siRNA ( si-NC) were transfected into AC16 cells , and the cells were divided into H2O2 treatment (H2O2 ) group , H2 O2 + si NC group , and H2O2 + si-MALAT group . Cell proliferation activity was detected by CCK-8 assay , cell apoptosis was assessed by TUNEL assay , and the expression levels of cleaved caspase-3 , Bcl-2 associated X protein (Bax ) , and B cell lymphoma-2 ( Bcl-2) were determined by Western blot.
Results :Compared to the Normal group , the expression of MALAT1 was upregulated and the expression of miR-181a was down regulated in the AMI group (P < 0.05) , and there was a negative correlation between MALAT1 and miR-181a expression. The lncBase online prediction database and dual-luciferase reporter assay results had proven that MAL AT1 could target and regulate the expression of miR 181 a. Compared to the H2O2 group, the H2O2 + si MALAT group showed increased cell viability (P < 0.05) , decreased TUNEL positive rate (P < 0.05) , decreased expres sion levels of cleaved caspase-3 and Bax (P < 0.05) , and increased expression level of Bcl-2 (P < 0.05) , while the H2O2 + si NC group showed no significant changes (P > 0.05) .
Conclusion : LncRNA MALAT1 expression is elevated in AMI patients , which could promote oxidative stress induced myocardial cell damage through targeted inhibition of miR-181a.
- Full text:202406261622456026lncRNA_MALAT1...化应激模型中的心肌细胞损伤_郑丽华.pdf
