Neurobehavioral effects of long term mild hypothermia combined with compound porcine cerebroside and ganglioside injection after traumatic brain injury in rats
10.19405/j.cnki.issn1000-1492.2024.03.013
- Author:
Wanyong Zhao
1
;
Shugang Xu
1
;
Jingjing Wang
2
;
Xiaohong Li
3
;
Hongtao Sun
2
Author Information
1. Dept of Neurosurgery , Qilu Hospital of Shandong University Dezhou Hospital , Dezhou 253014
2. Neurotrauma Repair Research Institute , Characteristic Medical Center of Peoples Armed Police Forces , Tianjin 300162
3. Institute of Medical Engineering and Translational Medicine , Tianjin University , Tianjin 300350
- Publication Type:Journal Article
- Keywords:
mild hypothermia;
compound porcine cerebroside and ganglioside inj ection;
traumatic brain inj ury;
neurobehavior;
neurons;
apoptosis
- From:
Acta Universitatis Medicinalis Anhui
2024;59(3):448-454
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the neurobehavioral effects of long term mild hypothermia (MHT) combined with compound porcine cerebroside and ganglioside inj ection (CPCGI) after traumatic brain inj ury (TBI) in rats and its mechanism.
Methods :36 healthy adult male SD rats were randomly divided into model group , MHT group , CPCGI group and MHT + CPCGI group . The TBI model was prepared using an electronically controlled cortical in j ury device . The rats in model group received an intraperitoneal inj ection of an equal amount of normal saline (NS , 2 ml/kg) and were treated at room temperature (37 ℃) for 48 hours . The rats in MHT group received an intraper itoneal inj ection of an equal amount of NS and were treated at a slightly low temperature (33.0 ±1 0) ℃ for 48 hours . The rats in CPCGI group received an intraperitoneal inj ection of an equal amount of CPCGI (0.6 ml/kg) and were treated at room temperature for 48 hours . The rats in MHT + CPCGI group received an intraperitoneal injection of an equal amount of CPCGI and were treated at a slightly low temperature for 48 hours. The sensorimotor function of rats was evaluated by modified Neurological Severity Score ( mNSS) . The motor and spatial memory a bilities of rats were detected by Morris water maze test , and the motor function of rats was evaluated by beam walk ing test (BWT) and inclined grid climbing test. The number of neurons in hippocampus was ob served by Nissl stai ning and immunofluorescence was used to detect the expression of doublecortin (DCX) and neuronal nuclear anti gen antibody (NeuN) . Western blot was used to ob serve the protein expression of B cell lymphoma-2 ( Bcl-2) , Bcl 2 associated X protein ( Bax ) and cysteine proteinase-3 ( Caspase-3) .
Results :Compared with MHT group and CPCGI group , MHT + CPCGI group had a lower mNNS score, shorter escape latency , higher times across the platform and the percentage of time in the target quadrant , higher BWT score and larger climbing angle , increased numbers of neurons , DCX and NeuN positive cells , increased Bcl-2 expression and decreased expression of Bax and Caspase-3 . (P < 0.05) .
Conclusion :Long-term mild hypothermia combined with CPCGI can effectively improve the neurological deficits of TBI rats by promoting nerve regeneration and inhibiting cell apoptosis , and provide potential strategies and basis for the clinical treatment of TBI .
- Full text:2024062614574684673长时程亚低温联合复方脑肽节...脑创伤大鼠的神经行为学影响_赵万勇.pdf
