The role of HMGB1 in trigeminal neuralgia
10.19405/j.cnki.issn1000-1492.2024.03.011
- Author:
Lian Shen
1
;
Beibei Wu
1
;
Liecheng Wang
2
;
Yuanyin Wang
1
Author Information
1. College and Hospital of Stoma tology , Anhui Medical University , Anhui Provincial Key Laboratory of Oral Disease Research , Hefei 230032
2. Dept of Physiology , School of Basic Medicine , Anhui Medical University , Hefei 230032
- Publication Type:Journal Article
- Keywords:
trigeminal neuralgia;
HMGB1;
glcyrrhizin;
suborbital nerve constriction surgery;
TLR4;
NF-κB
- From:
Acta Universitatis Medicinalis Anhui
2024;59(3):436-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To construct a rat model of trigeminal neuralgia ( TN) to explore the expression of high mobility group box-1 (HMGB1) in the trigeminal ganglion (TG) and the possible mechanism of HMGB1 effect on pain .
Methods :TN model was constructed by infraorbital nerve constriction and divided into operation group (CCI group) and Sham group , and the success of the model construction was determined through mechanical pain thresh old assessment. Real time fluorescence quantitative PCR ( RT-qPCR) and Western blot were used to detect high mobility group protein B1 (HMGB1) , Toll receptor 4 (TLR4) , and Nuclear Factor Kappa B(NF-κB) mRNA and protein expression in the ipsilateral trigeminal ganglion (TG) of the Sham and CCI rats . 50 mg/kg HMGB1 inhibi tor glycyrrhizin (GL) was inj ected intraperitoneally every day for two week s , and normal saline (NS) was used as control . The patients were divided into CCI group , CCI + NS group and CCI + GL group . HMGB1 , TLR4 , and NF- κB mRNA and protein expression in the ipsilateral trigeminal ganglion (TG) were detected by RT-qPCR and West ern blot in CCI group , CCI + NS group , and CCI + GL group .
Results :The mechanical threshold on the operated side of the rat continued to decrease (P < 0.05) , and mechanical pain threshold identification model was success fully constructed . After chronic compressive injury to the infraorbital nerve in rats , HMGB1 , TLR4 , and NF-κB mRNA and protein expression in TG on the operated side increased ( P < 0.05) ; After administration of HMGB1 inhibitor Glcyrrhizin , HMGB1 , TLR4 , NF-κB showed a decrease (P < 0.05) .
Conclusion :HMGB1 is associat ed with TN , and HMGB1 may be involved in the pathogenesis of TN through TLR4/NF-κB signaling pathway.
- Full text:2024062611514266998HMGB1在三叉神经痛中的作用_沈莲.pdf
