Effect of dihydroartemisinin on anti tumor immune response of CD8 + T cells induced by non small cell lung cancer cells
10.19405/j.cnki.issn1000-1492.2024.03.009
- Author:
Nannan Wang
1
;
Yu Liu
1
;
Huijuan Ling
1
;
Ke Niu
1
;
Yayu Zhu
1
;
Liwen Chen
2
Author Information
1. Dept of Clinical Laboratory , The Second Afiliated Hospital of Anhui Medical University , Hefei 230601
2. Dept of Blood Transfusion , The Second Afiliated Hospital of Anhui Medical University , Hefei 230601
- Publication Type:Journal Article
- Keywords:
dihydroartemisinin;
non small cell lung cancer;
tumor immunity;
CD8 + T cells;
perforin;
granzyme B
- From:
Acta Universitatis Medicinalis Anhui
2024;59(3):424-429
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the regulatory effect of artemisinin derivative dihydroartemisinin ( DHA) on anti-tumor immune function of CD8 + T cells induced by non-small cell lung cancer ( NSCLC) cells .
Methods:NSCLC A549 cells were divided into DMSO control group and DHA treatment group . A549 cells were treated with DMSO and DHA at different concentrations (25 , 50 and 100 μmol/L) , and the optimal concentration of DHA was selected to treat A549 cells for 0 , 24 , 48 and 72 h according to half maximal inhibitory concentrate (IC50 ) . CCK 8 method and colony formation test were used to detect the effect of DHA on the proliferation and colony formation ability of A549 cells . Peripheral blood mononuclear cells (PBMCs ) of healthy individuals were isolated by density gradient centrifugation . After monocytes were removed by adhesion method , A549 cells pretreated with mitomycin C were co cultured with PBMCs at 10:1 ratio . After 2 weeks , flow cytometry was used to detect the proportion of CD8 + T cells and the expression levels of perforin and granzyme B .
Results :Compared with the control group , the proliferation inhibition rates of A549 cells increased after treatment with 25 , 50 and 100 μmol/L DHA for 24 h (P < 0.01) . The IC50 of DHA on A549 cells was 46.26 μmol/L. According to IC50 concentration analysis , the inhibi tion rates of A549 cells treated with 50 μmol/L DHA for 0 , 24 , 48 and 72h were 1 53% , 53.50% , 63.84% and 69.91% , and the cells inhibition rates of A548 cells increased compared with the previous ob servation time point , namely 0 , 24 and 48 h (P < 0.01) . The colony formation assay showed that the colony formation number of A549 cells in DHA treated group decreased compared with the control group (P < 0.01) . Flow cytometry results showed that compared with the control group , the proportion of CD8 + T cells induced by A549 cells in the co-culture system and the proportion of CD8 + T cells expressing perforin and granzyme B were higher in DHA pretreatment group(P < 0.01) .
Conclusion :DHA inhibits the growth of NSCLC cells and promotes anti tumor immune response of CD8 + T cells induced by NSCLC cells .
- Full text:2024062611162236641双氢青蒿素对非小细胞肺癌细...T细胞抗肿瘤免疫应答的影响_王南楠.pdf
