Effect of miR 27a targeting regulation of SFRP1 on biological behavior of colorectal cancer
10.19405/j.cnki.issn1000-1492.2024.03.008
- Author:
Xueqin Sima
1
;
Yanting Su
2
;
Zhi Zeng
3
Author Information
1. Dept of Histology and Embryology ,School of Basic Medical Sciences , Xianning Medical College , Hubei University of Science and Tecnology , Xianning 437100
2. Dept of Biochemistry and Molecular Biology , School of Basic Medical Sciences , Xianning Medical College , Hubei University of Science and Tecnology , Xianning 437100
3. Dept of Pathology , Xianning Central Hospital , Xianning 437100
- Publication Type:Journal Article
- Keywords:
miR 27a;
SFRP1;
colorectal cancer;
Wnt4;
β catenin
- From:
Acta Universitatis Medicinalis Anhui
2024;59(3):418-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the expression of miR-27a in colorectal cancer cell , and to analyze the effect of its targeted regulation of ( Secreted Frizzled Related Protein , SFRP1) on the biological behavior of colorectal cancer cells .
Methods :Real time fluorescent quantitative PCR(qRT-PCR) was employed to examine the expres sion of miR-27a and SFRP1 mRNA in colorectal cancer tissues and adjacent normal tissues . Western blot was used to detect the expression of SFRP1 protein in colorectal cancer tissues and adjacent normal tissues . TargetScan soft ware and dual luciferase reporter gene test were used to detect the targeted regulation of miR-27a on SFRP1 . HCT116 cells were transfected with miR-27a mimic , miR-27a inhibitor and negtive control (NC) . The expression of miR-27a and SFRP1 mRNA in each group was determined by qRT-PCR . MTT colorimetry was performed to eval uate the proliferation of each group cells . Transwell assay was used to evaluate the cell invasion and migration ability . Meanwhile , the protein expression levels of SFRP1 , key factors Wnt4 and β-catenin in the Wnt/β catenin signaling pathway were determined by Western blot.
Results :Compared with adjacent normal tissues , miR-27a was highly expressed in colorectal cancer tissues , while SFRP1 was low expressed in colorectal cancer tissues ( P < 0.05) . Target Scan software and dual luciferase reporter gene test showed that miR-27a targeted SFRP1 . Compared with NC group , the expression of miR-27a of miR-27a mimic group increased , the proliferation , invasion and migration ability enhanced , the expression of SFRP1 protein decreased , while Wnt4 and β-catenin protein expression increased ( P < 0.05 ) . Compared with miR-27a mimic group , the expression of miR-27a of miR-27a inhibitor group decreased , the proliferation , invasion and migration ability reduced , the expression of SFRP1 protein in creased , while Wnt4 and β-catenin protein expression decreased( P < 0.05) .
Conclusion :miR-27a can target SFRP1 , inhibit the proliferation , invasion and migration of colorectal cancer cells , mainly by up regulating SFRP1 and blocking the downstream Wnt/β-catenin signaling pathway , which provides a new direction for clinical treatment.
- Full text:2024062611001528828miR-27a靶向调控SF...对结直肠癌生物学行为的影响_司马学琴.pdf
