Research progress on eukaryotic expression system of recombinant protein drugs
10.13200/j.cnki.cjb.004244
- VernacularTitle:重组蛋白药物真核表达系统的研究进展
- Author:
ZHANG Junhe
- Publication Type:Journal Article
- From:
Chinese Journal of Biologicals
2024;37(6):756-762
- CountryChina
- Language:Chinese
-
Abstract:
The recombinant protein drugs have the advantages of high activity,high specificity and low toxicity,which have a very broad application prospect in the world.At present,the recombinant therapeutic proteins in vitro are produced from prokaryotic and eukaryotic expression system in order to achieve efficient expression.In view of the advantages such as perfect post-translational modification and protein folding,eukaryotic expression system has been widely used in the production of recombinant protein drugs.There are four commonly used eukaryotic expression systems,including yeast,insect cell,mammalian cell and plant cell expression systems.In this paper,the characteristics,applications and prospects of several common eukaryotic expression systems are reviewed,in view to providing a reference for the selection of suitable expression systems for recombinant protein drugs and promoting the research and development of new drugs.
