Immunogenicity study of varicella-zoster virus Oka-7S strain

VernacularTitle:水痘-带状疱疹病毒Oka-7S株的免疫原性分析
Author: ZHANG Shujie
Publication Type:Journal Article
Keywords: Varicella-zoster virus(VZV); Enzyme-linked immunospot assay(ELISPOT); Fluorescent antibody to membrane antigen assay(FAMA); Immunogenicity
From: Chinese Journal of Biologicals 2024;37(6):660-665
CountryChina
Language:Chinese
Abstract: Objective To compare the immunogenicity of varicella-zoster virus(VZV)Oka-7S strain and VZV Oka strain.Methods Female BALB/c mice were subcutaneously immunized with VZV Oka-7S and Oka strains at high,medium and low doses(1 000,250 and 62.5 PFU/dose)for total two doses at an interval of 14 d,separately,with 12 mice in each group.The VZV-specific humoral immunity was evaluated based on fluorescent antibody to membrane antigen assay(FAMA),and the VZV-specific cellular immunity was evaluated by enzyme-linked immunospot assay(ELISPOT).Results Both VZV Oka-7S and VZV Oka strains induced specific humoral and cellular immune responses,and showed considerable immune effects;The level of anti-VZV specific IgG in serum of mice in Oka-7S-2.2 group increased significantly with the increase of immunization times and immunization doses.Conclusion The VZV Oka-7S strain had comparative immunogenicity with VZV Oka strain.