Effects of PATL1 on the proliferation and migration of gastric cancer cells and its mechanism of action
10.19405/j.cnki.issn1000-1492.2024.01.021
- Author:
Zeyu Xu
1
;
Zhiyong Lai
2
;
Yifan Ren
2
;
Feng Wu
2
;
Yuting Peng
1
;
Jun Xu
2
Author Information
1. Dept of Biochemistry and Molecular Biology，School of Basic Medicine，Shanxi Medical University，TaiYuan 030001
2. Dept of Hepatobiliary and Pancreatic Surgery，First Hospital of Shanxi Medical University，TaiYuan 030001
- Publication Type:Journal Article
- Keywords:
gastric cancer;
proliferation;
migration;
PATL1;
autophagy associated protein
- From:
Acta Universitatis Medicinalis Anhui
2024;59(1):133-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective :Objective
Methods :The expression levels of PATL1 in pancar- cinoma，gastric cancer and normal tissues were analyzed by TCGA database．The expression level of PATL1 in 40 human gastric cancer tissues and paired adjacent tissues was evaluated by quantitative real-time PCR (RT-qPCR) . The Kaplan-Meier Plotter database was used to analyze the prognosis of PATL1 in gastric cancer patients．The gas- tric cancer cell line AGS was transfected with PATL1 interference vector，and the interference effect was evaluated by RT-qPCR. The effects of PATL1 on the proliferation and migration of AGS were detected by cell counting kit-8 ( CCK-8) ，Transwell test and scratch healing test．The effects of interference with PATL1 on the expression of cel- lular-myelocytomatosis viral oncogene ( c-Myc) and autophagy related 7 ( ATG7) proteins in gastric cancer cells were detected by Western blot assay.
Results :RT-qPCR showed that the expression of PATL1 in human gastric cancer tissue was higher than that in normal gastric tissue (P＜0. 001) ，and PATL1 was correlated with the progno- sis of patients with enteric gastric cancer (P＜0. 000 1) ．After PATL1 was knocked down，the number of prolifera- ting and migrating gastric cancer cells decreased (P＜0. 05) ．Western blot test results showed that the expression level of ATG7 protein decreased after PATL1 was knocked down (P＜0. 05) ．
Conclusion :PATL1 may inhibit the proliferation and migration of gastric cancer cells through crosstalk with c-Myc and ATG7 .
- Full text:2024062509012447224PATL1对胃癌细胞增殖和迁移能力的影响及其作用机制_徐泽宇.pdf
