AAMP Promotes Osteosarcoma Cell Metastasis by Regulating YAP Signaling Pathway
10.3971/j.issn.1000-8578.2024.23.1034
- VernacularTitle:AAMP通过调节YAP信号通路促进骨肉瘤细胞转移的机制研究
- Author:
Qianrong DENG
1
,
2
;
Fangbiao ZHAN
1
,
3
;
Chaozheng XIE
4
;
Shuang XIANG
1
,
5
;
Jian CHEN
1
,
3
;
Yi YANG
1
,
6
Author Information
1. Postgraduate Training Base of Jinzhou Medical University, Chongqing University Three Gorges Hospital, Chongqing 404000, China
2. Health Management Center, Chongqing University Three Gorges Hospital, Chongqing 404000, China.
3. Department of Orthopedics, Chongqing University Three Gorges Hospital, Chongqing 404000, China.
4. Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
5. Department of Pathology, Chongqing University Three Gorges Hospital, Chongqing 404000, China.
6. Department of Hematology, Chongqing University Three Gorges Hospital, Chongqing 404000, China.
- Publication Type:BASICRESEARCH
- Keywords:
Osteosarcoma;
AAMP;
YAP;
CFL1;
Migration;
Invasion;
Pseudopodium
- From:
Cancer Research on Prevention and Treatment
2024;51(6):440-447
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the role of AAMP in osteosarcoma cells and explore the mechanism of invasion and metastasis of osteosarcoma cells regulated by AAMP through the YAP signaling pathway. Methods Public sequencing data analysis was used to explore the correlation between AAMP and osteosarcoma. q-PCR, Western blot, and immunohistochemistry were used to detect the expression levels of osteosarcoma cell-related molecules. CCK-8 was used to detect cell proliferation ability. Transwell and wound healing assays were used to detect the invasive and metastatic abilities of osteosarcoma cells. Immunofluorescence was used to detect the cell localization and expression levels of related molecules. Results High expression of AAMP was negatively correlated with the prognosis of patients with osteosarcoma (P<0.05), and the expression of AAMP in patients with metastatic osteosarcoma increased (P<0.05). Compared with the control group, the AAMP interference group showed significantly decreased migratory, invasive, and EMT activities (P<0.05). The expression of p-CFL1 reduced after the knockdown of AAMP, and the cell plate pseudopods decreased significantly (P<0.05). A positive correlation was found between the expression levels of AAMP and YAP in osteosarcoma cells (P<0.05). Interfering with YAP expression can affect the migration and invasion of osteosarcoma cells. Conclusion AAMP promotes osteosarcoma cell metastasis by regulating the YAP signaling pathway, suggesting that AAMP may be a key molecule in promoting invasion and metastasis of osteosarcoma.
