Effect of miR-141-3p targeting HMGB1 on LPS-induced A549 cell injury
10.19405/j.cnki.issn1000-1492.2024.01.014
- Author:
Guangwen Long
1
;
Qian Zhang
1
;
Xiulin Yang
1
;
Hongpeng Sun
1
;
Chunling Ji
1
Author Information
1. Dept of Emergency Medicine，Guizhou Provincial People＇s Hospital，Guiyang 550002
- Publication Type:Journal Article
- Keywords:
type Ⅱ alveolar epithelial cells;
A549;
lipopolysaccharide;
miR-141-3p;
high mobility group protein 1
- From:
Acta Universitatis Medicinalis Anhui
2024;59(1):85-91
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of miR-141-3p on LPS induced A549 cell injury by targeting high mobility group protein 1 (HMGB1) ．
Methods :A549 cells derived from type Ⅱ alveolar epithelial cells were taken as the study object，miR-141-3p mimics，mimics NC，HMGB1 gene overexpression plasmid (pcDNA3. 1-HMGB1) and empty Vector were transfected into A549 cells respectively or co-transfected，then 10 μg / ml LPS was used for 24 h．Cell proliferation activity was detected by cell counting kit-8 ( CCK-8) ．The activity of lactate dehydrogenase ( LDH) in the supernatant of cell culture was detected by colorimetry．The apoptosis level of each group was detec- ted by flow cytometry．The levels of interleukin (IL) -1 β , IL-6 and tumor necrosis factor α (TNF-α) were detected by enzyme-linked immunosorbent assay (Elisa) ．Dual luciferase reporter gene assay verified the targeted regulatory relationship between miR-141-3p and HMGB1 .
Results :After treatment with LPS ，the proliferative activity of A549 cells and the expression level of miR-141-3p decreased ( P ＜0. 05 ) ，the apoptosis rate increased ( P < 0. 05) ，the levels of IL-1 β , IL-6，TNF-α and the activity of LDH in supernatant increased (P＜0. 05) ．Overex- pression of miR-141-3p increased the proliferation activity of A549 cells treated with LPS (P ＜0. 05 ) ，and de- creased the apoptosis rate and the levels of IL-1 β , IL-6，TNF-α in cells and LDH activity in supernatant (P < 0. 05) ．However，overexpression of HMGB1 gene could reverse the ameliorative effect of miR-141-3p on LPS-in- duced A549 cell injury．Dual luciferase reporter gene experiment confirmed that HMGB1 was the downstream target gene of miR-141-3p．
Conclusion : miR-141-3p can inhibit LPS-induced apoptosis，reduce the expression level of inflammatory factors，and improve the damage of A549 cells，which may be related to the targeted regulation of HMGB1 expression.
- Full text:202406241643166525miR-141-3p靶向调...导的A549细胞损伤的影响_龙光文.pdf
