Analysis of drug resistance and pathogenicity of six strains of Klebsiella pneumoniae
10.19405/j.cnki.issn1000-1492.2024.01.012
- Author:
Chengyu Sui
1
,
2
;
Jiazhen Wang
3
,
4
;
Zhijun Zhang
5
;
Lili Zhang
5
;
Meng Lv
5
;
Dongsheng Zhou
5
;
Wenhui Yang
1
,
2
Author Information
1. School of Basic Medical Sciences，Anhui Medical University，Hefei 230032
2. State Key Laboratory of Pathogen and Biosecurity，Beijing Institute of Microbiology and Epidemiology，Academy of Military Medical Sciences，Beijing 100071
3. State Key Laboratory of Pathogen and Biosecurity，Beijing Institute of Microbiology and Epidemiology，Academy of Military Medical Sciences，Beijing 100071
4. Dept of Immunology of Basic Medical College，Guizhou Medical University，Guiyang 550025
5. State Key Laboratory of Pathogen and Biosecurity，Beijing Institute of Microbiology and Epidemiology，Academy of Military Medical Sciences，Beijing 100071
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
clinical isolates;
drug resistance;
pathogenicity;
viscosity
- From:
Acta Universitatis Medicinalis Anhui
2024;59(1):71-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the drug resistance and pathogenicity of six clinical isolates of Klebsiella pneu- moniae (Kp) ，and to provide a basis for prevention and treatment of Kp infection．
Methods :The six strains from different hospitals were isolated ，cultured ，and identified by species-specific gene khe． Their whole genome se- quences (WGS) were obtained using next-generation sequencing technology (NGS) ．Based on the WGS，the cap- sular serotypes，sequence types (ST) and drug-resistance genes of six strains were identified．The capsular sero- type genes and virulence genes were validated or identified using PCR. Broth microdilution tests were conducted to validate their drug susceptibility，and mice were challenged with Kp aerosols by MicroSprayer aerosolizer to evaluate their pathogenicity.
Results : The six strains were all serotype K2 but belonged to four ST types ( ST14 ，ST65， ST700，and ST86) ，and collectively carried six virulence genes and 23 drug-resistance genes．All the six strains were resistant to ampicillin，but only one strain was multidrug-resistant．Four strains exhibited high mucoid charac- teristics．Five strains could cause mortality in mice，which were preliminary identified as high virulence strains.
Conclusion : For the six Kp clinical isolates from different sources，only one strain named NY 13294 is both multi- drug-resistant and highly virulent，and other four highly virulent strains are resistant to one or two types of antibiot- ics.
- Full text:20240624162957597226株肺炎克雷伯菌的耐药性及致病性分析_隋成宇.pdf
