Effect of knockdown IGSF10 on proliferation，migration and invasion capacity of lung adenocarcinoma cells
10.19405/j.cnki.issn1000-1492.2024.01.008
- Author:
Lianyu Cheng
1
;
Beibei Ma
1
;
Yu Huang
2
;
Yanli Li
1
;
Zhongwei Zhang
3
;
Guangbin Ye
1
;
Bo Ling
1
Author Information
1. School of Basic Medical Sciences，Youjiang Medical University for Nationalities，Baise 533000
2. Nursing School, Youjiang Medical University for Nationalities，Baise 533000
3. School of Pharmacy，Youjiang Medical University for Nationalities，Baise 533000
- Publication Type:Journal Article
- Keywords:
IGSF10;
lung adenocarcinoma;
proliferation;
migration;
invasion
- From:
Acta Universitatis Medicinalis Anhui
2024;59(1):45-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of immunoglobulin gene superfamily 10 (IGSF10) on prolifera- tion，migration and invasion of lung adenocarcinoma cells．
Methods :ioinformatics was applied to study the ex- pression levels of IGSF10 in tumor tissues and normal tissues． Western blot and quantitative real-time PCR ( qPCR) were used to detect the expression level of IGSF10 in lung adenocarcinoma cell lines and normal lung epi- thelial cells．Knockdown of IGSF10，the effect of knockdown of IGSF10 on proliferation，migration and invasion of lung adenocarcinoma A549 cells was examined using cell counting kit-8 ( CCK-8) ，Transwell migration and inva- sion assay，scratch assay and plate cloning assay．The effects of knockdown of IGSF10 on the expression of invasion and migration-related genes in A549 cells were examined by Western blot and qPCR assays.
Results :IGSF10 ex- pression in lung adenocarcinoma tissues was lower than that in normal tissues (P ＜0. 05) ．IGSF10 expression in lung adenocarcinoma cell lines was lower than that in lung epithelial cells (P＜0. 05) ．Knockdown of IGSF10 pro- moted the ability of lung adenocarcinoma A549 cells to proliferate ，proliferation ，migration and invasion ( P < 0. 05) ．Knockdown of IGSF10 promoted the expression of regulatory epithelial-mesenchymal transition marker Neu- ral-cadherin (N-cadherin) and key transcription factors Snail family transcriptional repressor 1 (Snail) and Snail family transcriptional repressor 2 (Slug) (P＜0. 05) and inhibited the expression of Epithelial-cadherin (E-cad- herin) (P＜0. 05) ．
Conclusion : Knockdown of IGSF10 may promote proliferation，migration and invasion of lung adenocarcinoma cells through activation of Snail，Slug / E-cadherin signaling axis，and this result may provide a po- tential new target for clinical diagnosis and treatment of lung adenocarcinoma.
- Full text:2024062414595776849敲低IGSF10对肺腺癌细...增殖、迁移和侵袭能力的影响_程联钰.pdf
