Screening of stable amoxicillin resistant clones of Helicobacter pylori and detection of its gene mutations
10.19405/j.cnki.issn1000-1492.2024.01.007
- Author:
Qiudan Lu
1
,
2
,
3
;
Mengheng Mi
1
;
Guzhen Cui
1
,
4
;
Zhengrong Zhang
1
;
Xiaojuan Wu
1
;
Zhenghon Chen
1
Author Information
1. Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou，School of Basic Medical Science，Guizhou Medical University，Guiyang 550025
2. Dept of Laboratory，Qiandongnan Prefecture People '
3. s Hospital，Kaili 556000
4. Key Laboratory of Endemic and Ethnic Diseases， Ministry of Education，Key Laboratory of Medical Molecular Biology of Guizhou Province，Guizhou Medical University，Guiyang 550004
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
amoxicillin;
unstable resistance;
stable resistance
- From:
Acta Universitatis Medicinalis Anhui
2024;59(1):39-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the phenotype of amoxicillin ( AMX) unstable resistant Helicobacter pylori (Hp) evolving into AMX stable high level resistance and the detection of its mutated genes．
Methods :Using the frozen Hp strain H390 as the starting strain，the clones resistant to AMX were continuously cultured on the medium with increasing AMX concentration，and the minimum inhibitory concentration ( MIC) of the resistant clones was detected．After frozen at －80 ℃ for 3 months，the drug resistance was stable according to whether the MIC de- creased after frozen storage． Genome sequencing analysis and efflux pump inhibition assay were performed on cloned H390r and parental strain H390 with the highest AMX MIC value，and gene mutations associated with the high level AMX resistance obtained by H390r were detected and identified.
Results :Four AMX high level resistant clones were obtained by AMX screening with MICs of 12，32，64 and ≥ 256 mg / L ，respectively，and none of the MICs were altered after freezing at －80 ℃ . Compared to the parental strain H390，the AMX stable resistant clone H390r had mutations in several genes，including hefC encoding the RND efflux system，hopB and hopC encoding the pore proteins and ftsI encoding the penicillin binding protein ，which were associated with AMX resistance. H390r was substantially reduced in MIC to AMX in the presence of efflux pump inhibitors．
Conclusion :AMX can screen stable resistant clones from unstable resistant Hp．H390r had mutations in hefC，hopB，hopC，and ftsI asso- ciated with AMX resistance．These mutations may be the main reason why H390r acquired a stable high level of re- sistance to AMX.
- Full text:2024062414405041997幽门螺杆菌阿莫西林稳定耐药...隆的筛选及其基因突变的检测_陆秋丹.pdf
