Effects of miR-155 on apoptosis of chronic myeloid leukemia cells and expression of heat shock proteins
10.19405/j.cnki.issn1000-1492.2024.01.006
- Author:
Yingli Ren
1
;
Zhidong Chen
1
;
Beibei Sun
1
;
Jie Yao
1
;
Fan Ma
1
;
Qiang Zhou
1
Author Information
1. Dept of Clinical Laboratory，The Second Affiliated Hospital of Anhui Medical University，Hefei 230601
- Publication Type:Journal Article
- Keywords:
miR-155;
chronic myeloid leukemia;
drug resistance;
heat shock proteins;
cell proliferation;
apopto- sis
- From:
Acta Universitatis Medicinalis Anhui
2024;59(1):34-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To explore the effects of microRNA-155 (miR-155) on apoptosis of chronic myeloid leukemia ( CML) cells，and the influence of miRNA-155 regulating the expression of heat shock proteins ( HSP) 27， HSP60，HSP70．
Methods :Reverse transcription-quantitative real-time PCR ( RT-qPCR) was used to detect the expression levels of miR-155 in CML-resistant imatinib (IM) cell line K562-G and CML cell line K562 ．K562-G cells were infected with the lentivirus carrying miR-155 or the negative control lentivirus ，and they were named miR-155 group and control group．The effect of miR-155 on the proliferation of drug-resistant cells was detected by cell counting kit-8 ( CCK-8) method. RT-qPCR and Western blot were used to detect the effect of miR-155 on the expression of heat shock proteins HSP27，HSP60，HSP70．Flow cytometry was used to detect the percentage of cell apoptosis in miR-155 group and control group.
Results :ompared with K562 cells，miR-155 showed low expres- sion in K562-G cells (P ＜0. 05) ．The proliferation of miR-155 group cells decreased significantly from the 36th hour (P＜0. 05) ．Compared with the control group，in the miR-155 group，HSP60 and HSP70 increased (P < 0. 05) ，while HSP27 decreased (P＜0. 01) ．The apoptosis rate of miR-155 group was higher than that of control group (P＜0. 05) ．
Conclusion :miR-155 promotes the apoptosis of chronic myeloid leukemia cells，increases the expression of HSP60 and HSP70，and decreases the expression of HSP27 .
- Full text:2024062411243913856miR-155对慢性髓细胞...凋亡及热休克蛋白表达的影响_任影丽.pdf
