Two-sample Mendelian randomization analysis of the causal relationship between T．gondii infection and encephalitis
10.19405/j.cnki.issn1000-1492.2024.01.005
- Author:
Yifan Li
1
;
Shuyan Sheng
2
;
Mengyun Wu
1
;
Yongsheng Ji
3
;
Yong Yao
1
Author Information
1. Dept of Parasitology and Microbiology，School of Basic Medicine，Anhui Medical University，Hefei 230032
2. The First Clinical Medical College， Anhui Medical University，Hefei 230032
3. Division of Life Sciences and Medicine，University of Science and Technology of China，Hefei 230027
- Publication Type:Journal Article
- Keywords:
Mendelian randomization;
Toxoplasma gondii;
encephalitis
- From:
Acta Universitatis Medicinalis Anhui
2024;59(1):28-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective :Mendelian randomization analysis was used to explore the causal relationship of T．gondii in- fection and the cyst distribution and inflammation in brain tissue by immunohistochemistry．
Methods :Genome- wide association analysis data of T．gondii infection and encephalitis were obtained ，single nucleotide polymor- phisms (SNPs) were selected，Mendelian randomization analysis was conducted by inverse variance weighting，and the causal relationship between T．gondii infection and encephalitis was evaluated by OR value and 95% CI．Quality control was carried out by using heterogeneity test，horizontal multi-efficiency test and leave-one-out sensitivity test. Immunohistochemical staining was performed using brain sections of mice infected with tissue cysts of Wh6 strain for image analysis using Image J software.
Results :A total of 29 SNPs were associated with toxoplasmic encephalitis. The results of IVW method suggested that T．gondii infection made encephalitis risk 0. 98 times higher ( OR = 0. 98， 95% CI = 0. 76 to 1. 27) ，indicating no causal relationship between the two．The quality control results suggested that the selected SNPs were stable and reliable．Toxoplasma cysts were distributed in various parts of the brain tis- sue．
Conclusion : T．gondii infection and encephalitis are related，but there is no sufficient evidence to prove the causal relationship between the two.
- Full text:2024062411151821588弓形虫感染与脑炎因果关系的双样本孟德尔随机化分析_李伊凡.pdf
