The brain protective effects of ropivacaine on cerebral ischemia/reperfusion injury in rats and its influence on cGAS/STING pathway
10.19845/j.cnki.zfysjjbzz.2023.0032
- VernacularTitle:罗哌卡因对脑缺血/再灌注损伤大鼠的脑保护作用及cGAS/STING通路的影响
- Author:
Kai LIU
1
;
Shuai YUAN
1
Author Information
1. Department of Anesthesiology,The First Affiliated Hospital of Shaoyang University,Shaoyang 422000,China
- Publication Type:Journal Article
- Keywords:
Ropivacaine;
Cerebral ischemia-reperfusion;
Brain protection;
Cyclic adenosine-adenosine synthase/stimulator of interferon gene
- From:
Journal of Apoplexy and Nervous Diseases
2023;40(2):133-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the brain protective effects of ropivacaine on cerebral ischemia-reperfusion injury in rats and its influence on cyclic adenosine-adenosine synthase/stimulator of interferon gene (cGAS/STING) pathway.Methods SD rats were randomly divided into sham operation group,model group,low-dose ropivacaine group (0.075 mg/kg),medium-dose ropivacaine group(0.15 mg/kg),and high-dose ropivacaine group (0.30 mg/kg),positive control group(nimodipine,5 mg/kg),with 8 rats in each group,except for the sham operation group,the rats in the other groups were given suture method to construct a rat model of cerebral ischemia and reperfusion,and the neurological deficit scores of the rats in each group on the 0th,3rd,and 7th day of administration were measured;hematoxylin-eosin staining (HE) was used to detect the pathological changes of brain tissues in each group;2、3、5-Triphenyte-trazoliumchloride(TTC) method was used to detect cerebral infarction area; enzyme-linked immunosorbent (ELISA) was used to detect the levels of tumor necrosis factor(TNF-α),interleukin(IL-1β),IL-6,superoxide dismutase(SOD) and malondialdehyde (MDA) in brain tissue; qRT-PCR was used to detect the level of mitochondrial DNA (mtDNA);Western blotting was used to detect the expression of cGAS/STING pathway protein in the brain tissues.Results Compared with the sham operation group,the brain tissue of the rats in the model group was seriously damaged,cerebral infarction area,the nerve defect score,TNF-α,IL-1β,IL-6,MDA levels in brain tissue,Cytoplasmic mtDNA level,cGAS and STING proteins expression were significantly increased,and the SOD levels in brain tissue were significantly reduced (P<0.05);compared with those in the model group,the brain tissue damage of the rats in the ropivacaine groups and the positive control group was relieved,cerebral infarction area,the nerve defect score,TNF-α,IL-1β,IL-6,MDA levels in brain tissue,Cytoplasmic mtDNA level,cGAS,STING proteins expression were significantly reduced,and SOD levels in brain tissue were significantly increased (P<0.05). Conclusion Ropivacaine has a certain protective effect on cerebral ischemia-reperfusion injury,and can inhibit cGAS/STING pathway.
- Full text:202406172253408663The brain protective effects of ropivacaine on cerebral ischemia_reperfusion injury in rats and its influence on cGAS_STING pathway.pdf
