Tracking Intravenous Adipose-Derived Mesenchymal Stem Cells in a Model of Elastase-Induced Emphysema.
10.4046/trd.2014.77.3.116
- Author:
You Sun KIM
1
;
Ji Young KIM
;
Dong Myung SHIN
;
Jin Won HUH
;
Sei Won LEE
;
Yeon Mok OH
Author Information
1. Asan Institute for Life Science, Seoul, Korea. ymoh55@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Mesenchymal Stromal Cells;
Emphysema;
Cell Tracking;
Injections, Intravenous
- MeSH:
Adipose Tissue;
Animals;
Bone Marrow;
Cell Tracking;
Emphysema*;
Fluorescence;
Humans;
Injections, Intravenous;
Kidney;
Liver;
Lung;
Mesenchymal Stromal Cells*;
Mice;
Optical Imaging;
Pulmonary Disease, Chronic Obstructive;
Quantum Dots;
Real-Time Polymerase Chain Reaction;
Spleen
- From:Tuberculosis and Respiratory Diseases
2014;77(3):116-123
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Mesenchymal stem cells (MSCs) obtained from bone marrow or adipose tissue can successfully repair emphysematous animal lungs, which is a characteristic of chronic obstructive pulmonary disease. Here, we describe the cellular distribution of MSCs that were intravenously injected into mice with elastase-induced emphysema. The distributions were also compared to the distributions in control mice without emphysema. METHODS: We used fluorescence optical imaging with quantum dots (QDs) to track intravenously injected MSCs. In addition, we used a human Alu sequence-based real-time polymerase chain reaction method to assess the lungs, liver, kidney, and spleen in mice with elastase-induced emphysema and control mice at 1, 4, 24, 72, and 168 hours after MSCs injection. RESULTS: The injected MSCs were detected with QD fluorescence at 1- and 4-hour postinjection, and the human Alu sequence was detected at 1-, 4- and 24-hour postinjection in control mice (lungs only). Injected MSCs remained more in mice with elastase-induced emphysema at 1, 4, and 24 hours after MSCs injection than the control lungs without emphysema. CONCLUSION: In conclusion, our results show that injected MSCs were observed at 1 and 4 hours post injection and more MSCs remain in lungs with emphysema.
