A study of differentially expressed proteins in Parkinson disease based on label-free quantitative proteomics
10.19845/j.cnki.zfysjjbzz.2023.0146
- VernacularTitle:应用非标记定量蛋白组学研究帕金森病差异表达蛋白
- Author:
Yunfei LONG
1
,
2
,
3
;
Haibo CHEN
1
,
2
,
3
Author Information
1. Department of Neurology,Beijing Hospital
2. National Center of Gerontology
3. Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing 100730,China
- Publication Type:Journal Article
- Keywords:
Parkinson disease;
Label-free quantitative technology;
Proteomics
- From:
Journal of Apoplexy and Nervous Diseases
2023;40(7):636-640
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biomarkers for Parkinson disease(PD) by analyzing differentially expressed proteins in the serum of patients with PD. Methods A total of 24 patients with PD who were diagnosed in Beijing Hospital were enrolled,among whom 12 patients only had a medical history of PD(group P1) and the other 12 had underlying diseases such as hypertension and diabetes(group P2). A total of 24 individuals,matched for age and sex,who underwent physical examination were enrolled as control groups C1 and C2.Serum samples were collected,and after high-abundant proteins were removed,the label-free quantitative proteomics technique was used to measure the expression of proteins in serum. The double comparison method was used for comparison between groups P1 and C1 and between groups P2 and C2 to screen for differentially expressed proteins,and the proteins with a consistent changing trend(upregulation or downregulation) were identified as the differentially expressed proteins for PD,which were analyzed and interpreted by bioinformatics methods. Results Comparison between groups identified four differentially expressed proteins,i.e. PRG4,CFHR-3,ACTG1,and HIST2H2BF,among which PRG4 and CFHR-3 showed upregulated expression and ACTG1 and HIST2H2BF showed downregulated expression in the serum of PD patients,and a certain degree of interaction was observed. Conclusion Label-free quantitative proteomics can be used to identify the differentially expressed proteins in PD,which may have a certain value in the diagnosis of PD.
- Full text:2024061614173539060应用非标记定量蛋白组学研究帕金森病差异表达蛋白.pdf
