Expression and role of TLR4/MyD88 in the hippocampus of rats with post‐traumatic stress disorder induced by single prolonged stress
- VernacularTitle:TLR4/MyD88在单一延长应激诱导的PTSD大鼠海马中的表达及意义
- Author:
Yanan ZHAO
1
;
Juhua XIE
1
Author Information
- Publication Type:Journal Article
- Keywords: Post‐traumatic stress disorder; Single prolonged stress; Hippocampus; TLR4; MyD88
- From: Journal of Apoplexy and Nervous Diseases 2023;40(11):1021-1024
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the expression and role of Toll-like receptor 4 （TLR4）/myeloid differentiation factor 88 （MyD88） in the hippocampus of a rat model of post‐traumatic stress disorder （PTSD） induced by single prolonged stress （SPS）. Methods Forty-five rats were randomly assigned into control group （n = 15） or PTSD group （n = 30； 15 each were examined on days 4 and 7 after modeling，respectively）. PTSD was induced by SPS，which was recognized internationally. The Morris water maze test was carried out to measure the spatial memory of the rats. The morphological changes of the hippocampus were examined with Nissl staining. The expression of TLR4，MyD88，and downstream nuclear factor-kappa B （NF-κB） was determined by Western blot. Results Compared with the control group，the PTSD group showed a significantly prolonged mean escape latency （P<0.05）； sparsely arranged and lightly stained neurons and a reduced number of Nissl bodies within cytoplasm in the hippocampus； and significantly increased protein expression of TLR4，MyD88，and NF-κB on days 4 and 7 after SPS stimulation （all P<0.05）. Conclusion SPS can impair the spatial memory of rats，which may be associated with the up-regulation of TLR4/MyD88/NF-κB in the hippocampus. The activation of TLR4/MyD88 signaling may be one of important molecular mechanisms in the development of SPS-induced PTSD.
- Full text:2024061320145524853Expression and role of TLR4_MyD88 in the hippocampus of rats with post‐traumatic stress disorder induced by single prolonged stress.pdf
