HLA-B27 Subtypes in Korean Patients with Ankylosing Spondylitis: by polymerase chain reaction-sequence specific primers (PCR-SSP) method.
- Author:
Miwon HWANG
1
;
Yoon Jeong CHO
Author Information
1. Department of Clinical Pathology, Korea University Medical College, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
HLA-B27 subtypes;
Ankyosing spondylitis;
PCR-SSP
- MeSH:
Alleles;
Arthritis, Psoriatic;
Arthritis, Reactive;
Discrimination (Psychology);
Erythema Nodosum;
Genetic Predisposition to Disease;
HLA-B27 Antigen*;
Humans;
Major Histocompatibility Complex;
Polymerase Chain Reaction;
Spondylitis, Ankylosing*;
Uveitis, Anterior
- From:Korean Journal of Clinical Pathology
1999;19(1):92-97
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The class I major histocompatibility complex (MHC) antigen, HLA-B27 appears to be the major genetic susceptibility factor for ankylosing spondylitis (AS), anterior uveitis, and reactive arthritis, but the mechanism underlying the association remains unknown. HLA-B27 consists of eleven closely related alleles (B*2701-B*2711) which differ in a restricted number of nucleotide substitutions. The aim of this study was to investigate the frequency and the contribution of the HLA-B27 subtypes to AS. METHODS: Forty-six patients (36 AS, 4 anterior uveitis, and 2 psoriatic arthritis, 2 reactive arthritis, 1 erythema nodosum, 1 rheumatic valvular disease) were analysed. The polymerase chain reaction with sequence-specific primers (PCR-SSP) method was used to define B27 allele subtypes. The primers were specifically designed for the discrimination of HLA-B*2701-B*2711. RESULTS: Thirty-two out of forty-six patients were typed. Among them, 27 AS patients were typed. Only two subtypes were identified : 88.9% (24 out of 27) were typed as B*2705 and 11.1% (3 out of 27) were typed as B*2704. Other 5 non-AS patients ( 4 anterior uveitis & 1 psoriatic arthritis) were also typed as B*2705. CONCLUSIONS: No difference in the distribution of HLA-B27 subtypes between patients and healthy controls could be found (Fisher's exact test : P= 0.867, P>0.05). Any specific B27 subtypes don't appear to contribute to AS susceptibility.
