Evaluation of Genedia HBsAg Rapid and Genedia Anti-HBs Rapid for the Screening of HBsAg and Anti-HBs.
- Author:
Jiha OH
1
;
Think You KIM
;
Hyun Jung YOON
;
Hyoung Sik MIN
;
Hye Rim LEE
;
Tae Yeal CHOI
Author Information
1. Department of Clinical Pathology, Hanyang University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
HBsAg;
Anti-HBs;
Screening kit;
Genedia HBsAg Rapid;
Genedia Anti-HBs Rapid
- MeSH:
Antigens, Surface;
Chungcheongnam-do;
Emergencies;
Hemagglutination;
Hepatitis;
Hepatitis B Surface Antigens*;
Immunoenzyme Techniques;
Mass Screening*
- From:Korean Journal of Clinical Pathology
1999;19(1):114-117
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: We evaluated a rapid screening kit for the detection of hepatitis surface antigen (HBsAg) and antibody (anti-HBs) using an immunochromatographic method. MATERIALS AND METHODS: We selected 499 serum specimens for the evaluation. Each specimen was tested by enzyme immunoassay (EIA; Cobas Core, Roche, Switzerland), reverse passive hemagglutination (RPHA; Serodia HBs, Asan, Korea) for HBsAg, passive hemagglutination (PHA; Serodia Anti-HBs, Asan, Korea) for anti-HBs, and with the Genedia HBsAg and Anti-HBs Rapid (Green Cross Corp., Korea) kits. Results of each assay were compared with those of the EIA. RESULTS: The sensitivities and specificities of the Genedia HBsAg Rapid kit were 98.0% and 100%, and those for the Genedia Anti-HBs Rapid kit were 95.3% and 98.0%, respectively. These were higher than those for RPHA (96.0% and 100%), and PHA (83.2% and 96.0%). Concordance rates between EIA and Genedia HBsAg Rapid, Genedia Anti-HBs Rapid, RPHA, and PHA were 98.8%, 96.4%, 97.6%, and 88.4%, respectively. Extending the incubation time from the recommended 30 minutes to 2 hours increased the sensitivities of the Genedia kits. CONCLUSION: The Genedia HBsAg and Anti-HBs Rapid kits are simple, sensitive, and inexpensive assays suitable for screening or use in emergency situations.
