Construction of porous structure on the surface of PLLA/β-tricalcium scaffold and its effect on cell adhesion

Hui Zeng; Fang Guo; Shuo Huang; Ning Liu; Yayuan Guo; Yuqi Zhang; Changkui Liu

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Construction of porous structure on the surface of PLLA/β-tricalcium scaffold and its effect on cell adhesion

VernacularTitle:PLLA/β-TCP支架表面多孔结构的构建及其对细胞黏附的影响
Author: Hui ZENG ^{1
,

2
,

3} ; Fang GUO ^{1
,

2
,

3} ; Shuo HUANG ^{1
,

2
,

3} ; Ning LIU ^{1
,

2
,

3} ; Yayuan GUO ^{1
,

2
,

3} ; Yuqi ZHANG ⁴ ; Changkui LIU ^{1
,

2
,

3}
Author Information

1. Department of Stomatology, Xi’an Medical College, Xi’an 710021
2. Research Center of Tooth and Maxillofacial Tissue Regeneration and Restoration, Xi’an 710021
3. Engineering Research Center for Oral Biomaterials and Advanced Equipment, Universities of Shaanxi Province, Xi’an 710021
4. School of Stomatology, Lanzhou University, Lanzhou 730000, China
Publication Type:Journal Article
Keywords: PLLA/β-tricalcium (PLLA/β-TCP); 3D printing; bone tissue engineering scaffold; NaOH; cell adhesion
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(3):428-434
CountryChina
Language:Chinese
Abstract: 【Objective】 To construct a 3D printed PLLA/β-tricalcium (PLLA/β-TCP) bone tissue engineering scaffold surface porous structure through simple treatment with NaOH solution, increase the roughness and hydrophilicity of the scaffold, and promote cell adhesion on the scaffold surface. 【Methods】 The PLLA/β-TCP mesh scaffold was prepared by 3D printing melt deposition molding technology, and the scaffold was roughed by NaOH etching. The effects of NaOH concentration and time on the scaffold were observed according to the microstructure, energy spectrum, contact angle, mechanics, and cell adhesion of the scaffold. 【Results】 The PLLA/β-TCP composite scaffold constructed by melt deposition technology had a pre-set porous structure, and the pores were interconnected. After NaOH etching, a porous structure with both macroscopic and microscopic pores was formed. The increase in any of the NaOH concentration and time parameters would lead to the increase of pore diameter and surface roughness. When the NaOH treatment parameter was 0.1 mol/L (9 h), it could significantly reduce the water contact angle on the surface of the scaffold, and had no significant effect on the compressive strength of the scaffold. In vitro cell testing showed that the surface porous composite scaffold etched with NaOH had more advantages in the adhesion and proliferation of BMSCs. 【Conclusion】 Using NaOH to process 3D printing of PLLA/β-TCP bone tissue engineering scaffolds can effectively improve the surface morphology of the scaffold, and optimize its hydrophilicity and cell adhesion.