The role of mitochondrial unfolded protein response in palmitic acid-induced lipid accumulation in renal tubule epithelium in vitro
- VernacularTitle:线粒体未折叠蛋白反应在棕榈酸诱导肾小管上皮细胞脂质聚集中的作用
- Author:
Ruizhi WEN
1
;
Xiaogang DU
Author Information
- Keywords: mitochondrial unfolded protein reaction(UPRmt); palmitic acid(PA); human kidney 2(HK-2)cell; lipid accumulation; reactive oxygen species(ROS)
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(3):419-427
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of mitochondrial unfolded protein response(UPRmt)on lipid metabolism in human kidney 2(HK-2)cells.Methods Lipid accumulation was induced by palmitic acid(PA)in HK-2 cells.The cells were pretreated with siRNA or CDDO respectively.The intracellular lipid accumulation was observed by oil red staining;mitochondrial membrane potential(MMP)was measured by JC-1.The contents of reactive oxygen species(ROS)in mitochondria were measured by Mito-SOX,and the expressions of HSP60,LONP1,CLPP,ACOX1,PPARα,PGC1α and CPT1α were detected by Western blotting.Results PA induced lipid aggregation,MMP decrease,ROS generation in mitochondria and the decreased expression of UPRmt proteins(e.g.,HSP60 and LONP1)in HK-2 cells.Pretreatment of HK-2 cells with siRNA could aggravate lipid aggregation,MMP decrease and ROS generation induced by PA,and further decrease the expression of HSP 60and LONP1.Pretreatment of HK-2 cells with CDDO alleviated lipid aggregation,MMP decrease,ROS generation and decreased HSP60 and LONP1 expressions induced by PA.Conclusion Lipid aggregation in HK-2 cells induced by PA may be related to mitochondrial dysfunction and UPRmt has a protective effect on HK-2 cells in the process.
