Extracellular matrix stiffness induces CCL5 synthesis to enhance immunotherapy response in non-small cell lung cancer

Tian Zhang; Yaru Yang; Enyong Zhang; Hui Guo; Mengjie Liu

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Extracellular matrix stiffness induces CCL5 synthesis to enhance immunotherapy response in non-small cell lung cancer

VernacularTitle:细胞外基质刚度诱导CCL5合成以提高非小细胞肺癌免疫治疗响应
Author: Tian ZHANG ^{1
,

2} ; Yaru YANG ^{1
,

2} ; Enyong ZHANG ¹ ; Hui GUO ^{1
,

2} ; Mengjie LIU ^{1
,

2}
Author Information

1. Department of Medical Oncology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004
2. Bioinspired Engineering and Biomechanics Center, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
Publication Type:Journal Article
Keywords: non-small cell lung cancer (NSCLC); extracellular matrix stiffness; C-C motif chemokine ligand 5 (CCL5); immunotherapy response
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(3):396-404
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the mechanism of C-C motif chemokine ligand 5 (CCL5) modulation by extracellular matrix stiffness in non-small cell lung cancer (NSCLC) and to determine the effect of CCL5 on the immunotherapy response of NSCLC. 【Methods】 The correlation between extracellular matrix stiffness and CCL5 expression in NSCLC was analyzed with the TCGA database. Polyacrylamide hydrogels with different stiffness were designed according to the extracellular matrix stiffness of NSCLC, and H1299 cells responding to the mechanical loading of hydrogel matrix stiffness were subjected to transcriptome sequencing. High matrix stiffness was verified to promote the expression of CCL5 by using sequence. 【Results】 High extracellular matrix stiffness upregulated CCL5 expression, and interferon-γ mediated signaling pathway might be involved in the process. NSCLC patients with high CCL5 expression had a greater abundance of cytotoxic T-cells in tumor tissue and reacted favorably to anti-programmed cell death protein 1 treatment. 【Conclusion】 Increased extracellular matrix stiffness promotes CCL5 synthesis, and CCL5 enhances immunotherapy responsiveness in NSCLC by increasing cytotoxic T cell infiltration in tumor tissue.