Regulation of Local Immunoglobulin Expression by MZB1 in Chronic Rhinosinusitis with Nasal Polyps
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20240419.004
- VernacularTitle:慢性鼻窦炎伴鼻息肉中MZB1的表达及其对局部免疫球蛋白表达的影响
- Author:
Yan YAN
1
;
Zhaofeng XU
;
Yanran HUANG
Author Information
1. 中山大学附属第一医院耳鼻咽喉科医院,广东 广州,510080
- Keywords:
chronic rhinosinusitis;
chronic rhinosinusitis with nasal polyps;
immunoglobulin;
MZB1;
IgE
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2024;45(3):383-392
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]The heightened expression of local immunoglobulins is a significant pathological characteristic of chronic rhinosinusitis with nasal polyps(CRSwNP),particularly in the Asian population.This study is centered on ex-ploring the association between MZB1 and the localized aggregation of immunoglobulins in Asian individuals with CRSwNP.[Methods]Nasal polyp tissues obtained from 40 CRSwNP patients and inferior turbinates from 6 healthy controls under-went examination for both mRNA and protein levels.The assessments were conducted using polymerase chain reaction,lu-minex,and immunohistochemical staining.Statistical analyses,including one-way Anova(ANOVA),independent sam-ples t-test,and Pearson's correlation analysis,were employed for comprehensive data evaluation.[Results]The mRNA ex-pression levels of MZB1(P<0.01)and HSP90B1(P<0.01)were significantly higher in type 2 CRSwNP patients com-pared with those in healthy controls.Immunohistochemical staining revealed a significant increase in MZB1 protein expres-sion in type 2 CRSwNP.MZB1 demonstrated correlation with the expression of immunoglobulin E in nasal polyp tissues(P<0.01,r=0.52).Additionally,MZB1correlated with the expression ofIL-5(P<0.05,r=0.4)and IL-13(P<0.05,r=0.44)in nasal polyp tissues.Furthermore,MZB1showed correlation with the number of eosinophils in nasal polyp tis-sues(P<0.05,r=0.72).[Conclusion]The expression of MZB1 is notably elevated in Asian CRSwNP,particularly in type 2 CRSwNP,when compared with controls.MZB1expression correlates significantly with high IgE expression and dis-ease severity in nasal polyp tissues.
