Regulation of Local Immunoglobulin Expression by MZB1 in Chronic Rhinosinusitis with Nasal Polyps
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20240419.004
- VernacularTitle:慢性鼻窦炎伴鼻息肉中MZB1的表达及其对局部免疫球蛋白表达的影响
- Author:
Yan YAN
1
;
Zhaofeng XU
1
;
Yanran HUANG
1
Author Information
1. Division of Otorhinolaryngology，The First Affiliated Hospital，Sun Yat-sen University，Guangzhou 510080，China
- Publication Type:Journal Article
- Keywords:
chronic rhinosinusitis;
chronic rhinosinusitis with nasal polyps;
immunoglobulin;
MZB1;
IgE
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2024;45(3):383-392
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThe heightened expression of local immunoglobulins is a significant pathological characteristic of chronic rhinosinusitis with nasal polyps （CRSwNP）， particularly in the Asian population. This study is centered on exploring the association between MZB1 and the localized aggregation of immunoglobulins in Asian individuals with CRSwNP. MethodsNasal polyp tissues obtained from 40 CRSwNP patients and inferior turbinates from 6 healthy controls underwent examination for both mRNA and protein levels. The assessments were conducted using polymerase chain reaction， luminex， and immunohistochemical staining. Statistical analyses， including one-way Anova （ANOVA）， independent samples t-test， and Pearson's correlation analysis， were employed for comprehensive data evaluation. ResultsThe mRNA expression levels of MZB1（P < 0.01） and HSP90B1 （P < 0.01） were significantly higher in type 2 CRSwNP patients compared with those in healthy controls. Immunohistochemical staining revealed a significant increase in MZB1 protein expression in type 2 CRSwNP. MZB1 demonstrated correlation with the expression of immunoglobulin E in nasal polyp tissues （P < 0.01， r = 0.52）. Additionally，MZB1correlated with the expression ofIL-5（P < 0.05， r = 0.4） and IL-13 （P < 0.05， r = 0.44） in nasal polyp tissues. Furthermore，MZB1showed correlation with the number of eosinophils in nasal polyp tissues （P < 0.05， r = 0.72）. ConclusionThe expression of MZB1 is notably elevated in Asian CRSwNP， particularly in type 2 CRSwNP， when compared with controls.MZB1expression correlates significantly with high IgE expression and disease severity in nasal polyp tissues.
