Auxiliary diagnosis of visceral leishmaniasis with metagenomic next-generation sequencing: a case report
10.16250/j.32.1374.2023212
- VernacularTitle:宏基因组二代测序技术辅助诊断内脏利什曼病1例
- Author:
Weiping LI
1
;
Xinglei WANG
1
,
2
;
Li MA
3
;
Xinman DOU
1
,
4
;
Rui ZHAO
3
;
Yingqiao WANG
1
Author Information
1. School of Nursing, Lanzhou University, Lanzhou, Gansu 730000, China
2. Department of Hepatology, The Second Hospital of Lanzhou University, Langzhou, Gansu 730030, China
3. Department of Hepatology, The Second Hospital of Lanzhou University, Langzhou, Gansu 730030, China
4. Department of Nursing, The Second Hospital of Lanzhou University, China
- Publication Type:Journal Article
- Keywords:
Visceral leishmaniasis;
Metagenomic next-generation sequencing;
Auxiliary diagnosis;
Etiological detection
- From:
Chinese Journal of Schistosomiasis Control
2024;36(2):215-218
- CountryChina
- Language:Chinese
-
Abstract:
This case report summarizes the experience from diagnosis and treatment of a patient with repeated high fever, hepatosplenomegaly and pancytopenia. Following exclusion of bacterial, viral, fungal infections and hematological diseases, metagenomic next-generation sequencing of the patient’s peripheral blood revealed Leishmania infantum infection, and rK39 rapid diagnostic test showed positive for anti-Leishmania antibody, while microscopic examination of bone marrow smears identified Leishmania amastigotes. Therefore, the case was definitively diagnosed as visceral leishmaniasis, and given anti-infective treatment with sodium antimony gluconate and hormone, hepatoprotection, elevation of white blood cell counts and personalized nursing. Then, the case was cured and discharged from hospital. Metagenomic next-generation sequencing is of great value in etiological detection of fever patients with unknown causes, which deserves widespread clinical applications.
