Prevalence of Echinococcus infections in small rodents in Yushu City, Qinghai Province in 2023
10.16250/j.32.1374.2024007
- VernacularTitle:2023年青海省玉树市小型啮齿类动物 棘球属绦虫感染调查
- Author:
Gengcheng HE
1
;
Shusheng WU
2
;
Xianglan QIN
2
;
Jilong MA
2
;
Tingjun YU
2
;
Chengxi SONG
2
;
Xiaojin MO
1
,
3
;
Xiao MA
4
;
Jianfeng BA
5
;
Guirong ZHENG
6
;
Bin JIANG
1
;
Tian TIAN
1
;
Shijie YANG
1
;
Ting ZHANG
1
,
3
,
7
;
Xiaonong ZHOU
1
Author Information
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Health Commission Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai 200025, China
2. Yushu Tibetan Autonomous Prefecture Center for Disease Control and Prevention, Qinghai Province, China
3. Tibet Autonomous Region Center for Disease Control and Prevention, National Health Commission Key Laboratory of Echinococcosis Prevention and Control, China
4. Qinghai Institute for Endemic Disease Control and Prevention, China
5. Shiqu County Center for Disease Control and Prevention, Sichuan Province, China
6. School of Life Sciences, Inner Mongolia University, China
7. School of Life Sciences, Inner Mongolia University, China
- Publication Type:Journal Article
- Keywords:
Echinococcosis;
Echinococcus shiquicus;
Plateau pika;
Qinghai vole;
Cytochrome c oxidase 1;
Yushu City
- From:
Chinese Journal of Schistosomiasis Control
2024;36(2):169-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the prevalence of Echinococcus infections in small rodents around human residential areas in Yushu City, Qinghai Province in 2023, so as to provide insights into precision echinococcosis control. Methods One or two quadrats, each measuring 50 m × 50 m, were randomly assigned in Shanglaxiu Township and Longbao Township, Yushu City, Qinghai Province on June 2023, respectively, and 300 plate-type mouse traps, each measuring 12.0 cm × 6.5 cm, were assigned in each quadrat. Small rodents were captured during the period between 10 : 00 and 18 : 00 each day for 4 days. Then, all captured small rodents were identified and dissected, and liver specimens with suspected Echinococcus infections were subjected to pathological examinations. The Echinococcus cytochrome c oxidase 1 (cox1) gene was amplified using PCR assay, and the sequence of the amplified product was aligned to that was recorded in the GenBank to characterize the parasite species. In addition, a phylogenetic tree of Echinococcus was generated based on the cox1 gene sequence using the neighbor-joining method. Results A total of 236 small rodents were captured in Shanglaxiu and Longbao townships, Yushu City, including 65 Qinghai voles and 51 plateau pikas in Shanglaxiu Township, and 62 Qinghai voles and 58 plateau pikas in Longbao Township, and there was no significant difference in the constituent ratio of small rodents between the two townships (χ2 = 0.294, P > 0.05). Seven plateau pikas and 12 Qinghai voles were suspected to be infected with Echinococcus by dissection, and pathological examinations showed unclear structure of hepatic lobules and disordered hepatocyte arrangement in livers of small rodents suspected of Echinococcus infections. PCR assay identified E. shiquicus DNA in 7 Qinghai voles, which were all captured from Shanglaxiu Township. Phylogenetic analysis showed that the cox1 gene sequence of Echinococcus in small rodents was highly homologous to the E. shiquicus cox1 gene sequence reported previously. Conclusion Plateau pika and Qinghai vole were predominant small rodents around human residential areas in Yushu City, Qinghai Province in 2023, and E. shiquicus infection was detected in Qinghai voles.
