Investigation of tick - borne Rickettsia in selected areas of Liupanshui City, Guizhou Province in 2023
10.16250/j.32.1374.2023167
- VernacularTitle:2023年贵州省六盘水市部分地区蜱传立克次体调查
- Author:
Danni LI
1
,
2
,
3
;
Ya LI
3
,
4
;
Yonghui YU
5
;
Xuan OUYANG
5
;
Xiaolu XIONG
5
;
Shan JIN
1
;
Jun JIAO
5
Author Information
1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Animal Clinical Diagnosis and Treatment Technology, Ministry of Agriculture, Hohhot, Inner Mongolia 010018, China
2. Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China
3. Co-first authors
4. Department of Nephrology, People’s Hospital of Liupanshui City, Liupanshui, Guizhou 553000, China
5. Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China
- Publication Type:Journal Article
- Keywords:
Rhipicephalus microplus;
Rickettsia;
Candidatus Rickettsia jingxinensis;
Coxiella burnetii;
Coxiella-like endosymbiont;
Liupanshui City
- From:
Chinese Journal of Schistosomiasis Control
2024;36(2):154-158
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the prevalence of tick-borne rickettsial infections in selected areas of Liupanshui City, Guizhou Province, 2023, so as to provide insights into the management of tick-borne rickettsioses in the city. Methods Ticks were captured from the body surface of bovines and sheep in Gaoxing Village, Dashan Township, Liupanshui City, Guizhou Province during the period between April and June, 2023, and tick species were identified using morphological and molecular biological techniques. In addition, tick-borne Rickettsia was identified using a nested PCR assay, including spotted fever group rickettsiae (SFGR), Coxiella spp., Anaplasma spp., Ehrlichia spp., and Orientia spp., and positive amplified fragments were sequenced and aligned with known sequences accessed in the GenBank database. Results A total of 200 ticks were collected and all tick species were identified as Rhipicephalus microplus. Nestle PCR assay combined with sequencing identified ticks carrying Candidatus Rickettsia jingxinensis (40.50%), Coxiella burnetii (1.50%), and Coxiella-like endosymbionts (27.00%), and Anaplasma spp., Ehrlichia spp. or Orientsia spp. was not detected. Conclusions R. microplus carried Candidatus R. jingxinensis, C. burnetii, and Coxiella-like endosymbionts in selected areas of Liupanshui City, Guizhou Province. Intensified monitoring of tickborne rickettsial infections is needed in livestock and humans to reduce the damages caused by rickettsioses.
