UPLC-QTOF mass spectrometry detection of four endocrine disrupting chemicals (Methyl Paraben, 2,4-Dichlorophenoxyacetic acid, Monobutyl Phthalate, and Bisphenol A) in urine of Filipino women
https://doi.org/10.47895/amp.vi0.9007
- Author:
Francisco M. Heralde III
1
;
Zaidy T. Martin
1
;
Maria Stephanie Fay S. Cagayan
2
;
Ma. Esterlita V. Uy
3
;
Paulyn Jean R. Ubial
4
;
Michael C. Velarde
5
;
Erlidia F. Llamas-Clark
6
Author Information
1. Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila
2. Department of Pharmacology and Toxicology, College of Medicine, University of the Philippines Manila
3. Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines Manila
4. College of Public Health, University of the Philippines Manila
5. Institute of Biology, College of Science, University of the Philippines Diliman
6. Department of Obstetrics and Gynecology, Philippine General Hospital, University of the Philippines Manila
- Publication Type:Journal Article
- Keywords:
Bisphenol A, 2,4-Dichlorophenoxyacetic acid;
Methyl Paraben;
Monobutyl Phthalate;
UPLC-QTOF
- MeSH:
Endocrine Disruptors
- From:
Acta Medica Philippina
2024;58(Early Access 2024):1-10
- CountryPhilippines
- Language:English
-
Abstract:
Background and Objective:Endocrine Disrupting Chemicals (EDCs) are ubiquitously found as low-level contaminants and pose serious threat to women’s health. EDCs may result in various reproductive disorders, fetal birth and developmental abnormalities, and endocrine and metabolic disorders. EDCs can be detected in body fluids of exposed individuals including blood and urine. This study aimed to detect four EDCs — Methyl Paraben (MP), 2,4-Dichlorophenoxyacetic acid (2,4-D), Monobutyl Phthalate (MBP), and Bisphenol A (BPA) in urine samples of women using Ultra-Performance Liquid Chromatography – Quadrupole Time-of-Flight (UPLC-QTOF) mass spectrometry.
Methods:Sequential steps of enzymatic deconjugation, liquid-liquid extraction, solid phase extraction, and liquid chromatography separation and mass spectrometry detection were optimized in urine samples. The method was used to analyze 70 urine samples from women of reproductive age.
Results:The sample preparation method showed a recovery ranging from 86.6% (MBP) to 100 % (2,4-D). The method demonstrated limits of quantitation ranging from 1.52 ng/m(MP) to 6.46 ng/mL(2,4D). Intra-day precisions expressed as relative standard deviation were all below 15% while accuracy was shown to range from 67.10% (2,4-D) to 102.39% (MBP). MP was detected in nine samples (12.86%) with a geometric mean value of 10.15 ng/ml (range: 3.62-52.39 ng/ml). MBP was detected in 68 samples (97.14%) with a geometric mean value of 97.62 ng/ml (range: 15.32-698.18 ng/ml). BPA was detected only once (9.58 ng/ml) while 2, 4-D was not detected in all samples.
Conclusion:A UPLC-QTOF mass spectrometry method to detect four EDCs at parts per billion level (ng/ml) was adapted and applied for analysis of urine samples. This method can find applicability in routine testing of clinical specimens as well as surveillance and other epidemiological studies.
- Full text:20240607191957463529007-Other-157555-1-10-20240517 (1).pdf
