Distribution of CD4+CD25+ T cells and graft-versus-host disease in human hematopoietic stem cell transplantation.
10.3345/kjp.2008.51.12.1336
- Author:
Dae Hyoung LEE
1
;
Nak Gyun CHUNG
;
Dae Chul JEONG
;
Bin CHO
;
Hack Ki KIM
Author Information
1. Department of Pediatrics, College of Medicine, Hallym University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Regulatory T cell;
Graft-versus-fost Disease;
Allogeneic hematopoietic stem cell transplantation
- MeSH:
Cell Count;
Child;
Flow Cytometry;
Graft vs Host Disease;
Hematopoietic Stem Cell Transplantation;
Hematopoietic Stem Cells;
Humans;
T-Lymphocytes;
T-Lymphocytes, Regulatory;
Tissue Donors;
Transplants
- From:Korean Journal of Pediatrics
2008;51(12):1336-1341
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study aimed to determine the frequencies of CD4+CD25+ T cells in donor graft and peripheral blood CD4+CD25+ T cells in recipients after hematopoietic stem cell transplantation (HSCT) and their association with graft-versus-host disease (GVHD). METHODS: Seventeen children who underwent HSCT were investigated. CD4+CD25+ T cells in samples from donor grafts and recipient peripheral blood were assessed by flow cytometry at 1 and 3 months after transplantation. RESULTS: CD4+CD25+ T cell frequencies in the grafts showed no significant difference between patients with and without acute GVHD (0.90% vs. 1.06%, P=0.62). Absolute CD4+CD25+ T cell number in grafts were lower in patients with acute GVHD than in those without acute GVHD (6.18x10(5)/kg vs. 25.85x10(5)/kg, P=0.09). Patients without acute GVHD showed a significant decrease in peripheral blood CD4+CD25+ T cell percentage at 3 months compared to those at 1 month after HSCT (2.11% vs. 1.43%, P=0.028). However, in patients with acute GVHD, CD4+CD25+ T cell percentage at 3 months was not different from the corresponding percentage at 1 month after HSCT (2.47% vs. 2.30%, P=0.5). CONCLUSION: The effect of frequencies of CD4+CD25+ T cells in donor grafts on acute GVHD after HSCT could not be identified, and the majority of peripheral blood CD4+CD25+ T cells in patients who underwent HSCT may be activated T cells related to acute GVHD rather than regulatory T cells. Further studies with additional markers for regulatory T cells are needed to validate our results.
