Dexamethasone differentiates NG108-15 cells through cyclooxygenase1 induction.
- Author:
Hyeon Soo KIM
1
;
Minseok SONG
;
Euikyung KIM
;
Sung Ho RYU
;
Pann Ghill SUH
Author Information
1. Department of Life Science Division of Molecular and Life Science Pohang University of Science and Technology San 31 Hyoja-dong, Nam-gu, Pohang Kyungbuk 790-784, Korea. pgs@postech.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cell differentiation;
dexamethasone;
neurites;
prostaglandin-endoperoxide synthase;
prostaglandins indomethacin
- MeSH:
Animals;
Anti-Inflammatory Agents/pharmacology;
Cell Differentiation/*drug effects;
Cyclooxygenase Inhibitors/pharmacology;
Dexamethasone/*pharmacology;
Dinoprostone/metabolism;
Enzyme Induction;
Hybrid Cells;
Indomethacin/pharmacology;
Isoenzymes/*biosynthesis;
Mice;
Prostaglandin-Endoperoxide Synthase/*biosynthesis;
Rats;
Tumor Cells, Cultured
- From:Experimental & Molecular Medicine
2003;35(3):203-210
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cyclooxygenase (COX) is a key enzyme in the conversion of arachidonic acid into prostanoids which participate in various cellular functions including apoptosis, mitogenesis, inflammation, immune modulation and differentiation. Moreover, the synthetic glucocorticoid, dexamethasone has immune modulating and anti-inflammatory effects in vivo. Recently, dexamethasone was found to enhance retinoic acid-induced neuronal differentiation. In this study, we investigated the mechanisms of dexamethasone-mediated neuronal differentiation. Immunoblotting and morphological analysis demonstrated that dexamethasone induced neuronal differentiation through COX 1 induction. This phenomenon was inhibited by indomethacin, a COX inhibitor. In addition, the addition of exogenous prostaglandin E2 (PGE2), a substance produced by the COX-mediated pathway, triggered neurite outgrowth of cells treated with COX inhibitor. Taken together, COX 1 appears to play an important role in dexamethasone-mediated neuronal differentiation.
