Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles
10.4103/apjtb.apjtb_779_23
- Author:
Hoibin Jeong
1
;
Dong-joo Lee
2
;
Sung-Pil Kwon
3
,
4
,
5
;
SeonJu Park
1
;
Song-Rae Kim
1
;
Seung Hyun Kim
6
;
Jae-Il Park
7
;
Deug-chan Lee
5
;
Kyung-Min Choi
8
;
WonWoo Lee
8
;
Ji-Won Park
8
;
Bohyun Yun
8
;
Su-Hyeon Cho
7
;
Kil-Nam Kim
7
Author Information
1. Seoul Center, Korea Basic Science Institute, Seoul 02841, Republic of Korea
2. Chuncheon Center, Korea Basic Science Institute, Chuncheon 24341, Republic of Korea
3. Research &
4. Development Center, Chungdam CDC JNPharm LLC., Chuncheon 24341, Republic of Korea
5. Department of Biomedical Technology, Kangwon National University, Chuncheon 24341, Republic of Korea
6. Yonsei Institute of Pharmaceutical Sciences, College of Pharmacy, Yonsei University, Incheon 21983, Republic of Ko
7. Gwangju Center, Korea Basic Science Institute, Gwangju 61751, Republic of Korea
8. Honam National Institute of Biological Resources, 99, Gohadoan-gil, Mokpo-si, Jeollanam-do, 58762, Republic of Korea
- Publication Type:Journal Article
- Keywords:
Catalpa bignonioides;
Skeletal muscle;
Cell proliferation;
Mitochondria;
Energy metabolism;
C2C12
- From:Asian Pacific Journal of Tropical Biomedicine
2024;14(2):47-54
- CountryChina
- Language:English
-
Abstract:
Objective: To evaluate the effects of Catalpa bignonioides fruit extract on the promotion of muscle growth and muscular capacity in vitro and in vivo. Methods: Cell viability was measured using the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide assay. Cell proliferation was assessed using a 5-bromo-2’-deoxyuridine (BrdU) assay kit. Western blot analysis was performed to determine the protein expressions of related factors. The effects of Catalpa bignonioides extract were investigated in mice using the treadmill exhaustion test and whole-limb grip strength assay. Chemical composition analysis was performed using high-performance liquid chromatography (HPLC). Results: Catalpa bignonioides extract increased the proliferation of C2C12 mouse myoblasts by activating the Akt/mTOR signaling pathway. It also induced metabolic changes, increasing the number of mitochondria and glucose metabolism by phosphorylating adenosine monophosphate-activated protein kinase. In an in vivo study, the extract-treated mice showed improved motor abilities, such as muscular endurance and grip strength. Additionally, HPLC analysis showed that vanillic acid may be the main component of the Catalpa bignonioides extract that enhanced muscle strength. Conclusions: Catalpa bignonioides improves exercise performance through regulation of growth and metabolism in skeletal muscles, suggesting its potential as an effective natural agent for improving muscular strength.
- Full text:20240201.pdf
