Capsosiphon fulvescens suppresses LPS-stimulated inflammatory responses by suppressing TLR4/NF-κB activation in RAW264.7 murine macrophages
10.4103/apjtb.apjtb_865_23
- Author:
Seon Yeong Ji
1
,
2
;
EunJin Bang
1
,
2
;
Hyun Hwangbo
1
,
2
;
Min Yeong Kim
1
,
2
;
Da Hye Kim
1
,
2
;
Su Hyun Hong
1
,
2
;
Shin- Hyung Park
3
;
Chang-Young Kwon
4
;
Gi-Young Kim
5
;
You-Jin Jeon
5
;
Suengmok Cho
6
;
Yung Hyun Choi
1
,
2
Author Information
1. Department of Biochemistry, Dong-eui University College of Korean Medicine, Busan 47227, Republic of Korea
2. Basic Research Laboratory for the Regulation of Microplastic-Mediated Diseases and Anti-Aging Research Center, Dong-eui University, Busan 47227, Republic of Korea
3. Department of Pathology, Dong-eui University College of Korean Medicine, Busan 47227, Republic of Korea
4. Department of Oriental Neuropsychiatry, Dong-eui University College of Korean Medicine, Busan 47227, Republic of Korea
5. Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea
6. Department of Food Science and Technology/Institute of Food Science, Pukyong National University, Busan 48513, Republic of Korea
- Publication Type:Journal Article
- Keywords:
Capsosiphon fulvescens;
Inflammation;
Oxidative stress;
NF-κB;
Nrf2;
TLR4
- From:Asian Pacific Journal of Tropical Biomedicine
2024;14(3):115-126
- CountryChina
- Language:English
-
Abstract:
Objective: To evaluate the effects of Capsosiphon fulvescens (C. fulvescens) ethanolic extract on inflammation in lipopolysaccharide (LPS)-induced RAW296.7 macrophages. Methods: The protective effects of C. fulvescens ethanolic extract on LPS-induced inflammation in RAW264.7 macrophages were assessed using biochemical analysis, including enzyme-linked immunosorbent assay, quantitative reverse transcription-polymerase chain reaction, and Western blot analysis. To examine reactive oxygen species (ROS) production, flow cytometry analysis, and immunofluorescence staining were used. Furthermore, the modulatory effect of C. fulvescens ethanolic extract on NF-κB activation was investigated. Results: C. fulvescens ethanolic extract significantly attenuated LPS-induced levels of pro-inflammatory cytokines and notably reduced the secretion and mRNA levels of LPS-mediated matrix metalloproteinases. In addition, C. fulvescens ethanolic extract decreased ROS production and suppressed the TLR4/NF-κB signaling pathway. Conclusions: C. fulvescens ethanolic extract alleviates inflammation as well as oxidative stress by modulating the TLR4/NF-κB signaling in LPS-induced RAW264.7 macrophages. C. fulvescens can be used as a potential therapeutic agent to suppress inflammation and oxidative stress-associated diseases.
- Full text:20240304.pdf
