Ethyl acetate fraction of Sargassum pallidum extract attenuates particulate matterinduced oxidative stress and inflammation in keratinocytes and zebrafish
10.4103/apjtb.apjtb_921_23
- Author:
Chul Wook Kim
1
;
Ji-Won Park
2
;
Bohyun Yun
2
;
WonWoo Lee
2
;
Kyung-Min Choi
2
;
Seung-Hong Lee
1
,
3
Author Information
1. Department of Medical Science, Soonchunhyang University, Asan 31538, Republic of Korea
2. Honam National Institute of Biological Resources, 99, Gohadoan-gil, Mokpo-si, Jeollanam-do 58762, Republic of Korea
3. Department of Pharmaceutical Engineering, Soonchunhyang University, Asan 31538, Republic of Korea
- Publication Type:Journal Article
- Keywords:
Particulate matter;
Inflammation;
Oxidative stress;
Sargassum pallidum;
Ethyl acetate fraction;
Zebrafish
- From:Asian Pacific Journal of Tropical Biomedicine
2024;14(4):137-146
- CountryChina
- Language:English
-
Abstract:
Objective: To evaluate the effect of the ethyl acetate fraction derived from Sargassum pallidum extract against particulate matter (PM)-induced oxidative stress and inflammation in HaCaT cells and zebrafish. Methods: HaCaT cells and zebrafish were used to evaluate the protective effects of the ethyl acetate fraction of Sargassum pallidum extract against PM-induced oxidative stress and inflammation. The production of nitric oxide (NO), intracellular ROS, prostaglandin E2 (PGE2), and pro-inflammatory cytokines, and the expression levels of COX-2, iNOS, and NF-κB were evaluated in PM-induced HaCaT cells. Furthermore, the levels of ROS, NO, and lipid peroxidation were assessed in the PM-exposed zebrafish model. Results: The ethyl acetate fraction of Sargassum pallidum extract significantly decreased the production of NO, intracellular ROS, and PGE2 in PM-induced HaCaT cells. In addition, the fraction markedly suppressed the levels of pro-inflammatory cytokines and inhibited the expression levels of COX-2, iNOS, and NF-κB. Furthermore, it displayed remarkable protective effects against PM-induced inflammatory response and oxidative stress, represented by the reduction of NO, ROS, and lipid peroxidation in zebrafish. Conclusions: The ethyl acetate fraction of Sargassum pallidum extract exhibits a protective effect against PM-induced oxidative stress and inflammation both in vitro and in vivo and has the potential as a candidate for the development of pharmaceutical and cosmeceutical products.
- Full text:20240401.pdf
