Exploring Effect of Concentration on Quantitative Accuracy of QAMS by Taking Ginsenosides as an Example
10.13422/j.cnki.syfjx.20241161
- VernacularTitle:以人参皂苷为例探讨浓度对一测多评法定量准确性的影响
- Author:
Xi CHEN
1
;
Shasha KONG
1
;
Yuntao DAI
1
Author Information
1. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
- Publication Type:Journal Article
- Keywords:
multicomponent quantitative analysis;
quantitative analysis of multi-components by single marker（QAMS）;
concentration difference;
ginsenoside;
quantitative accuracy;
ultra performance liquid chromatography（UPLC）;
Ginseng Radix et Rhizoma
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(13):184-191
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the influence of concentration ratio（CR） between the internal reference and target components on the quantitative accuracy of quantitative analysis of multi-components by single marker（QAMS） by taking ginsenosides as an example. MethodUltra performance liquid chromatography（UPLC） was employed， the contents of nine components in Ginseng Radix et Rhizoma（ginsenosides Rg1， Re， Rf， Rh1， Rb1， Rc， Rb2， Rb3， Rd） were determined by external standard method（ES）. Using ginsenoside Rg1 as the internal reference， the contents of the remaining 8 ginsenosides were determined by QAMS， and the quantitative results were compared with those of ES to evaluate the quantitative accuracy of the established QAMS. According to the contents of these 9 ginsenosides， the simulated sample solutions with different CRs of ginsenoside Rg1 to ginsenosides Rf， Rb2， Rd were prepared with the reference substance（CR=100∶1， 10∶1， 5∶1， 2∶1， 1∶1， 0.5∶1， 0.25∶1）， in order to validate the effect of the CRs between the internal reference and other components on the quantitative accuracy of the QAMS. ResultThe results of ginsenosides Re， Rf， Rb1， Rc， Rb2 calculated by the two methods were the same with the relative standard deviation（RSD）<3%， however， the results of ginsenosides Rh1， Rb3 and Rd calculated by the two methods were different with RSDs of 7.06%-9.61%. According to the result of the simulated sample solution， the difference between the calculated results of ES and QAMS was large when the CR between the internal reference（ginsenoside Rg1） and other components was 5 or 10 or even higher. ConclusionThe quantitative error of QAMS will increase when the CR between the quantitative component and the internal reference is too large， so it is suggested that when establishing the QAMS， the components with close concentration to the internal reference should be selected for quantification.
